Introduction to Mass Spectrometry-Based Proteomics

Rune Matthiesen, Jakob Bunkenborg

Research output: Contribution to journalArticle

Abstract

Mass spectrometry, a technology to determine the mass of ionized molecules and biomolecules, is increasingly applied for the global identification and quantification of proteins. Proteomics applies mass spectrometry in many applications, and each application requires consideration of analytical choices, instrumental limitations and data processing steps. These depend on the aim of the study and means of conducting it. Choosing the right combination of sample preparation, MS instrumentation, and data processing allows exploration of different aspects of the proteome. This chapter gives an outline for some of these commonly used setups and some of the key concepts, many of which later chapters discuss in greater depth. Understanding and handling mass spectrometry data is a multifaceted task that requires many user decisions to obtain the most comprehensive information from an MS experiment. Later chapters in this book deal in-depth with various aspects of the process and how different tools addresses the many analytical challenges. This chapter revises the basic concept in mass spectrometry (MS)-based proteomics.

Original languageEnglish
Pages (from-to)1-58
Number of pages58
JournalMethods In Molecular Biology (Clifton, N.J.)
Volume2051
DOIs
Publication statusPublished - 1 Jan 2020

Fingerprint

Proteomics
Mass Spectrometry
Proteome
Technology
Proteins

Keywords

  • Data formats
  • Mass spectrometry
  • Proteomics
  • Sample preparation

Cite this

@article{cc3d1ab848334a4aa250e54e3809f1a5,
title = "Introduction to Mass Spectrometry-Based Proteomics",
abstract = "Mass spectrometry, a technology to determine the mass of ionized molecules and biomolecules, is increasingly applied for the global identification and quantification of proteins. Proteomics applies mass spectrometry in many applications, and each application requires consideration of analytical choices, instrumental limitations and data processing steps. These depend on the aim of the study and means of conducting it. Choosing the right combination of sample preparation, MS instrumentation, and data processing allows exploration of different aspects of the proteome. This chapter gives an outline for some of these commonly used setups and some of the key concepts, many of which later chapters discuss in greater depth. Understanding and handling mass spectrometry data is a multifaceted task that requires many user decisions to obtain the most comprehensive information from an MS experiment. Later chapters in this book deal in-depth with various aspects of the process and how different tools addresses the many analytical challenges. This chapter revises the basic concept in mass spectrometry (MS)-based proteomics.",
keywords = "Data formats, Mass spectrometry, Proteomics, Sample preparation",
author = "Rune Matthiesen and Jakob Bunkenborg",
year = "2020",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9744-2_1",
language = "English",
volume = "2051",
pages = "1--58",
journal = "Methods In Molecular Biology (Clifton, N.J.)",
issn = "1064-3745",
publisher = "Humana Press",

}

Introduction to Mass Spectrometry-Based Proteomics. / Matthiesen, Rune; Bunkenborg, Jakob.

In: Methods In Molecular Biology (Clifton, N.J.), Vol. 2051, 01.01.2020, p. 1-58.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Introduction to Mass Spectrometry-Based Proteomics

AU - Matthiesen, Rune

AU - Bunkenborg, Jakob

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Mass spectrometry, a technology to determine the mass of ionized molecules and biomolecules, is increasingly applied for the global identification and quantification of proteins. Proteomics applies mass spectrometry in many applications, and each application requires consideration of analytical choices, instrumental limitations and data processing steps. These depend on the aim of the study and means of conducting it. Choosing the right combination of sample preparation, MS instrumentation, and data processing allows exploration of different aspects of the proteome. This chapter gives an outline for some of these commonly used setups and some of the key concepts, many of which later chapters discuss in greater depth. Understanding and handling mass spectrometry data is a multifaceted task that requires many user decisions to obtain the most comprehensive information from an MS experiment. Later chapters in this book deal in-depth with various aspects of the process and how different tools addresses the many analytical challenges. This chapter revises the basic concept in mass spectrometry (MS)-based proteomics.

AB - Mass spectrometry, a technology to determine the mass of ionized molecules and biomolecules, is increasingly applied for the global identification and quantification of proteins. Proteomics applies mass spectrometry in many applications, and each application requires consideration of analytical choices, instrumental limitations and data processing steps. These depend on the aim of the study and means of conducting it. Choosing the right combination of sample preparation, MS instrumentation, and data processing allows exploration of different aspects of the proteome. This chapter gives an outline for some of these commonly used setups and some of the key concepts, many of which later chapters discuss in greater depth. Understanding and handling mass spectrometry data is a multifaceted task that requires many user decisions to obtain the most comprehensive information from an MS experiment. Later chapters in this book deal in-depth with various aspects of the process and how different tools addresses the many analytical challenges. This chapter revises the basic concept in mass spectrometry (MS)-based proteomics.

KW - Data formats

KW - Mass spectrometry

KW - Proteomics

KW - Sample preparation

UR - http://www.scopus.com/inward/record.url?scp=85072603641&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9744-2_1

DO - 10.1007/978-1-4939-9744-2_1

M3 - Article

VL - 2051

SP - 1

EP - 58

JO - Methods In Molecular Biology (Clifton, N.J.)

JF - Methods In Molecular Biology (Clifton, N.J.)

SN - 1064-3745

ER -