Introduction to biomolecular nuclear magnetic resonance and metals

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is a key technique for the structural and functional characterization of biological molecules. This chapter introduces the reader to the basics of the technique and progresses to specific features that originate from the presence of metals and their unpaired electrons in the molecules to be analyzed. This is done in a gradual fashion to accompany the demands of characterizing increasingly complex molecules. A few strategies to illustrate how paramagnetic molecules can be studied experimentally are presented. The description of NMR spectroscopy made here is targeted towards students or researchers unfamiliar with NMR and interested in the applications of the technique relevant for biomolecular work involving metalloproteins or interactions between proteins and metal ions.

Original languageEnglish
Title of host publicationPractical Approaches to Biological Inorganic Chemistry
PublisherElsevier
Pages155-199
Number of pages45
ISBN (Electronic)9780444642257
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • Chemical exchange
  • Chemical shift
  • In-cell nmr
  • Magnetization relaxation
  • NMR spectroscopy
  • Paramagnetism
  • Residual dipolar couplings

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