Introduction to Biomolecular NMR and Metals

Nuclear Magnetic Resonance (NMR) spectroscopy is nowadays a key technique in the structural and functional characterisation of biological molecules. This chapter brings the reader from the basics of the technique to specific features related to the presence of metals and their unpaired electrons. This is done in a gradual fashion to accompany the demands of increasingly complex molecules. The description of NMR spectroscopy made here is targeted towards students or researchers unfamiliar with NMR and interested in the applications of the technique relevant for biomolecular work involving metalloproteins or interactions between proteins and metal ions.