Intra-clade metabolomic profiling of MAR4 Streptomyces from the Macaronesia Atlantic region reveals a source of anti-biofilm metabolites

Anelize Bauermeister, Florbela Pereira, Inês R. Grilo, Camila C. Godinho, Marisa Paulino, Vanessa Almeida, Leonardo Gobbo-Neto, Alejandra Prieto-Davó, Rita G. Sobral, Norberto P. Lopes, Susana P. Gaudêncio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The search for new and effective strategies to reduce bacterial biofilm formation is of utmost importance as bacterial resistance to antibiotics continues to emerge. The use of anti-biofilm agents that can disrupt recalcitrant bacterial communities can be an advantageous alternative to antimicrobials, as their use does not lead to the development of resistance mechanisms. Six MAR4 Streptomyces strains isolated from the Madeira Archipelago, at the unexplored Macaronesia Atlantic ecoregion, were used to study the chemical diversity of produced hybrid isoprenoids. These marine actinomycetes were investigated by analysing their crude extracts using LC–MS/MS and their metabolomic profiles were compared using multivariate statistical analysis (principal component analysis), showing a separation trend closely related to their phylogeny. Molecular networking unveiled the presence of a class of metabolites not previously described from MAR4 strains and new chemical derivatives belonging to the napyradiomycin and marinone classes. Furthermore, these MAR4 strains produce metabolites that inhibit biofilm formation of Staphylococcus aureus and Marinobacter hydrocarbonoclasticus. The anti-biofilm activity of napyradiomycin SF2415B3 (1) against S. aureus was confirmed.

Original languageEnglish
Pages (from-to)1099-1112
Number of pages14
JournalEnvironmental Microbiology
Volume21
Issue number3
DOIs
Publication statusPublished - 1 Mar 2019

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Metabolomics
Streptomyces
metabolomics
Biofilms
biofilm
metabolite
metabolites
Staphylococcus aureus
Marinobacter hydrocarbonoclasticus
Marinobacter
Bacterial Drug Resistance
Actinobacteria
isoprenoid
ecoregion
isoprenoids
Terpenes
ecoregions
networking
Phylogeny
Principal Component Analysis

Cite this

Bauermeister, Anelize ; Pereira, Florbela ; Grilo, Inês R. ; Godinho, Camila C. ; Paulino, Marisa ; Almeida, Vanessa ; Gobbo-Neto, Leonardo ; Prieto-Davó, Alejandra ; Sobral, Rita G. ; Lopes, Norberto P. ; Gaudêncio, Susana P. / Intra-clade metabolomic profiling of MAR4 Streptomyces from the Macaronesia Atlantic region reveals a source of anti-biofilm metabolites. In: Environmental Microbiology. 2019 ; Vol. 21, No. 3. pp. 1099-1112.
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abstract = "The search for new and effective strategies to reduce bacterial biofilm formation is of utmost importance as bacterial resistance to antibiotics continues to emerge. The use of anti-biofilm agents that can disrupt recalcitrant bacterial communities can be an advantageous alternative to antimicrobials, as their use does not lead to the development of resistance mechanisms. Six MAR4 Streptomyces strains isolated from the Madeira Archipelago, at the unexplored Macaronesia Atlantic ecoregion, were used to study the chemical diversity of produced hybrid isoprenoids. These marine actinomycetes were investigated by analysing their crude extracts using LC–MS/MS and their metabolomic profiles were compared using multivariate statistical analysis (principal component analysis), showing a separation trend closely related to their phylogeny. Molecular networking unveiled the presence of a class of metabolites not previously described from MAR4 strains and new chemical derivatives belonging to the napyradiomycin and marinone classes. Furthermore, these MAR4 strains produce metabolites that inhibit biofilm formation of Staphylococcus aureus and Marinobacter hydrocarbonoclasticus. The anti-biofilm activity of napyradiomycin SF2415B3 (1) against S. aureus was confirmed.",
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Intra-clade metabolomic profiling of MAR4 Streptomyces from the Macaronesia Atlantic region reveals a source of anti-biofilm metabolites. / Bauermeister, Anelize; Pereira, Florbela; Grilo, Inês R.; Godinho, Camila C.; Paulino, Marisa; Almeida, Vanessa; Gobbo-Neto, Leonardo; Prieto-Davó, Alejandra; Sobral, Rita G.; Lopes, Norberto P.; Gaudêncio, Susana P.

In: Environmental Microbiology, Vol. 21, No. 3, 01.03.2019, p. 1099-1112.

Research output: Contribution to journalArticle

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AU - Bauermeister, Anelize

AU - Pereira, Florbela

AU - Grilo, Inês R.

AU - Godinho, Camila C.

AU - Paulino, Marisa

AU - Almeida, Vanessa

AU - Gobbo-Neto, Leonardo

AU - Prieto-Davó, Alejandra

AU - Sobral, Rita G.

AU - Lopes, Norberto P.

AU - Gaudêncio, Susana P.

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N2 - The search for new and effective strategies to reduce bacterial biofilm formation is of utmost importance as bacterial resistance to antibiotics continues to emerge. The use of anti-biofilm agents that can disrupt recalcitrant bacterial communities can be an advantageous alternative to antimicrobials, as their use does not lead to the development of resistance mechanisms. Six MAR4 Streptomyces strains isolated from the Madeira Archipelago, at the unexplored Macaronesia Atlantic ecoregion, were used to study the chemical diversity of produced hybrid isoprenoids. These marine actinomycetes were investigated by analysing their crude extracts using LC–MS/MS and their metabolomic profiles were compared using multivariate statistical analysis (principal component analysis), showing a separation trend closely related to their phylogeny. Molecular networking unveiled the presence of a class of metabolites not previously described from MAR4 strains and new chemical derivatives belonging to the napyradiomycin and marinone classes. Furthermore, these MAR4 strains produce metabolites that inhibit biofilm formation of Staphylococcus aureus and Marinobacter hydrocarbonoclasticus. The anti-biofilm activity of napyradiomycin SF2415B3 (1) against S. aureus was confirmed.

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DO - 10.1111/1462-2920.14529

M3 - Article

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JO - Environmental Microbiology

JF - Environmental Microbiology

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