Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function

Ahmed El Motiam, Santiago Vidal, Carlos F de la Cruz-Herrera, Sabela Da Silva-Álvarez, Maite Baz-Martínez, Rocío Seoane, Anxo Vidal, Manuel S Rodríguez, Dimitris P Xirodimas, Ana Sofia Carvalho, Hans Christian Beck, Rune Matthiesen, Manuel Collado, Carmen Rivas

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ribosomal protein L11 (RPL11) integrates different types of stress into a p53-mediated response. Here, we analyzed the impact of the ubiquitin-like protein SUMO on the RPL11-mouse double-minute 2 homolog-p53 signaling. We show that small ubiquitin-related modifier (SUMO)1 and SUMO2 covalently modify RPL11. We find that SUMO negatively modulates the conjugation of the ubiquitin-like protein neural precursor cell-expressed developmentally downregulated 8 (NEDD8) to RPL11 and promotes the translocation of the RP outside of the nucleoli. Moreover, the SUMO-conjugating enzyme, Ubc9, is required for RPL11-mediated activation of p53. SUMOylation of RPL11 is triggered by ribosomal stress, as well as by alternate reading frame protein upregulation. Collectively, our data identify SUMO protein conjugation to RPL11 as a new regulator of the p53-mediated cellular response to different types of stress and reveal a previously unknown SUMO-NEDD8 interplay.-El Motiam, A, Vidal, S., de la Cruz-Herrera, C. F., Da Silva-Álvarez, S., Baz-Martínez, M., Seoane, R., Vidal, A., Rodríguez, M. S., Xirodimas, D. P., Carvalho, A. S., Beck, H. C., Matthiesen, R., Collado, M., Rivas, C. Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function.

Original languageEnglish
Article numberfj201800341RR
Pages (from-to)643-651
Number of pages9
JournalFaseb Journal
Volume33
Issue number1
Early online date19 Jul 2018
DOIs
Publication statusPublished - Jan 2019

Fingerprint

Sumoylation
Ubiquitin
Ubiquitins
Small Ubiquitin-Related Modifier Proteins
Down-Regulation
Reading Frames
Protein Precursors
ribosomal protein L11
Up-Regulation
Chemical activation

Keywords

  • ARF
  • NEDD8
  • p53
  • SUMO

Cite this

El Motiam, A., Vidal, S., de la Cruz-Herrera, C. F., Da Silva-Álvarez, S., Baz-Martínez, M., Seoane, R., ... Rivas, C. (2019). Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function. Faseb Journal, 33(1), 643-651. [fj201800341RR]. https://doi.org/10.1096/fj.201800341RR
El Motiam, Ahmed ; Vidal, Santiago ; de la Cruz-Herrera, Carlos F ; Da Silva-Álvarez, Sabela ; Baz-Martínez, Maite ; Seoane, Rocío ; Vidal, Anxo ; Rodríguez, Manuel S ; Xirodimas, Dimitris P ; Carvalho, Ana Sofia ; Beck, Hans Christian ; Matthiesen, Rune ; Collado, Manuel ; Rivas, Carmen. / Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function. In: Faseb Journal. 2019 ; Vol. 33, No. 1. pp. 643-651.
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abstract = "The ribosomal protein L11 (RPL11) integrates different types of stress into a p53-mediated response. Here, we analyzed the impact of the ubiquitin-like protein SUMO on the RPL11-mouse double-minute 2 homolog-p53 signaling. We show that small ubiquitin-related modifier (SUMO)1 and SUMO2 covalently modify RPL11. We find that SUMO negatively modulates the conjugation of the ubiquitin-like protein neural precursor cell-expressed developmentally downregulated 8 (NEDD8) to RPL11 and promotes the translocation of the RP outside of the nucleoli. Moreover, the SUMO-conjugating enzyme, Ubc9, is required for RPL11-mediated activation of p53. SUMOylation of RPL11 is triggered by ribosomal stress, as well as by alternate reading frame protein upregulation. Collectively, our data identify SUMO protein conjugation to RPL11 as a new regulator of the p53-mediated cellular response to different types of stress and reveal a previously unknown SUMO-NEDD8 interplay.-El Motiam, A, Vidal, S., de la Cruz-Herrera, C. F., Da Silva-{\'A}lvarez, S., Baz-Mart{\'i}nez, M., Seoane, R., Vidal, A., Rodr{\'i}guez, M. S., Xirodimas, D. P., Carvalho, A. S., Beck, H. C., Matthiesen, R., Collado, M., Rivas, C. Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function.",
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El Motiam, A, Vidal, S, de la Cruz-Herrera, CF, Da Silva-Álvarez, S, Baz-Martínez, M, Seoane, R, Vidal, A, Rodríguez, MS, Xirodimas, DP, Carvalho, AS, Beck, HC, Matthiesen, R, Collado, M & Rivas, C 2019, 'Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function' Faseb Journal, vol. 33, no. 1, fj201800341RR, pp. 643-651. https://doi.org/10.1096/fj.201800341RR

Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function. / El Motiam, Ahmed; Vidal, Santiago; de la Cruz-Herrera, Carlos F; Da Silva-Álvarez, Sabela; Baz-Martínez, Maite; Seoane, Rocío; Vidal, Anxo; Rodríguez, Manuel S; Xirodimas, Dimitris P; Carvalho, Ana Sofia; Beck, Hans Christian; Matthiesen, Rune; Collado, Manuel; Rivas, Carmen.

In: Faseb Journal, Vol. 33, No. 1, fj201800341RR, 01.2019, p. 643-651.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function

AU - El Motiam, Ahmed

AU - Vidal, Santiago

AU - de la Cruz-Herrera, Carlos F

AU - Da Silva-Álvarez, Sabela

AU - Baz-Martínez, Maite

AU - Seoane, Rocío

AU - Vidal, Anxo

AU - Rodríguez, Manuel S

AU - Xirodimas, Dimitris P

AU - Carvalho, Ana Sofia

AU - Beck, Hans Christian

AU - Matthiesen, Rune

AU - Collado, Manuel

AU - Rivas, Carmen

PY - 2019/1

Y1 - 2019/1

N2 - The ribosomal protein L11 (RPL11) integrates different types of stress into a p53-mediated response. Here, we analyzed the impact of the ubiquitin-like protein SUMO on the RPL11-mouse double-minute 2 homolog-p53 signaling. We show that small ubiquitin-related modifier (SUMO)1 and SUMO2 covalently modify RPL11. We find that SUMO negatively modulates the conjugation of the ubiquitin-like protein neural precursor cell-expressed developmentally downregulated 8 (NEDD8) to RPL11 and promotes the translocation of the RP outside of the nucleoli. Moreover, the SUMO-conjugating enzyme, Ubc9, is required for RPL11-mediated activation of p53. SUMOylation of RPL11 is triggered by ribosomal stress, as well as by alternate reading frame protein upregulation. Collectively, our data identify SUMO protein conjugation to RPL11 as a new regulator of the p53-mediated cellular response to different types of stress and reveal a previously unknown SUMO-NEDD8 interplay.-El Motiam, A, Vidal, S., de la Cruz-Herrera, C. F., Da Silva-Álvarez, S., Baz-Martínez, M., Seoane, R., Vidal, A., Rodríguez, M. S., Xirodimas, D. P., Carvalho, A. S., Beck, H. C., Matthiesen, R., Collado, M., Rivas, C. Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function.

AB - The ribosomal protein L11 (RPL11) integrates different types of stress into a p53-mediated response. Here, we analyzed the impact of the ubiquitin-like protein SUMO on the RPL11-mouse double-minute 2 homolog-p53 signaling. We show that small ubiquitin-related modifier (SUMO)1 and SUMO2 covalently modify RPL11. We find that SUMO negatively modulates the conjugation of the ubiquitin-like protein neural precursor cell-expressed developmentally downregulated 8 (NEDD8) to RPL11 and promotes the translocation of the RP outside of the nucleoli. Moreover, the SUMO-conjugating enzyme, Ubc9, is required for RPL11-mediated activation of p53. SUMOylation of RPL11 is triggered by ribosomal stress, as well as by alternate reading frame protein upregulation. Collectively, our data identify SUMO protein conjugation to RPL11 as a new regulator of the p53-mediated cellular response to different types of stress and reveal a previously unknown SUMO-NEDD8 interplay.-El Motiam, A, Vidal, S., de la Cruz-Herrera, C. F., Da Silva-Álvarez, S., Baz-Martínez, M., Seoane, R., Vidal, A., Rodríguez, M. S., Xirodimas, D. P., Carvalho, A. S., Beck, H. C., Matthiesen, R., Collado, M., Rivas, C. Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function.

KW - ARF

KW - NEDD8

KW - p53

KW - SUMO

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DO - 10.1096/fj.201800341RR

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VL - 33

SP - 643

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JO - Faseb Journal

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El Motiam A, Vidal S, de la Cruz-Herrera CF, Da Silva-Álvarez S, Baz-Martínez M, Seoane R et al. Interplay between SUMOylation and NEDDylation regulates RPL11 localization and function. Faseb Journal. 2019 Jan;33(1):643-651. fj201800341RR. https://doi.org/10.1096/fj.201800341RR