Interactions of vanadium(V)-citrate complexes with the sarcoplasmic reticulum calcium pump

Manuel Aureliano, Teresa Tiago, Ricardo M. C. Gândara, Andrea Sousa, A. Moderno, M. Kaliva, A. Salifoglou, Rui O. Duarte, José J. G. Moura

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Abstract

Among the biotargets interacting with vanadium is the calcium pump from the sarcoplasmic reticulum (SR). To this end, initial research efforts were launched with two vanadium(V)-citrate complexes, namely (NH4) 6[V2O4(C6H4O 7)2] • 6H2O and (NH4) 6[V2O2(O2)2(C 6H4O7)2] • 4H2O, potentially capable of interacting with the SR calcium pump by combining kinetic studies with 51V NMR spectroscopy. Upon dissolution in the reaction medium (concentration range: 4-0.5 mM), both vanadium(V):citrate (VC) and peroxovanadium(V):citrate (PVC) complexes are partially converted into vanadate oligomers. A 1 mM solution of the PVC complex, containing 184 μM of the PVC complex, 94 μM oxoperoxovanadium(V) (PV) species, 222 μM monomeric (V1), 43 μM dimeric (V2) and 53 μM tetrameric (V4) species, inhibits Ca 2+ accumulation by 75 %, whereas a solution of the VC complex of the same vanadium concentration, containing 98 μM of the VC complex, 263 μM monomeric (V1), 64 μM dimeric (V2) and 92 μM tetrameric (V4) species inhibits the calcium pump activity by 33 %. In contrast, a 1 mM metavanadate solution, containing 460 μM monomeric (V1), 90.2 μM dimeric (V2) and 80 μM tetrameric (V4) species, has no effect on Ca2+ accumulation. The NMR signals from the VC complex (-548.0 ppm), PVC complex (-551.5 ppm) and PV (-611.1 ppm) are broadened upon SR vesicle addition (2.5 mg/ml total protein). The relative order for the half width line broadening of the NMR signals, which reflect the interaction with the protein, was found to be V4 > PVC > VC > PV > V2 = V1 = 1, with no effect observed for the V1 and V2 signals. Putting it all together the effects of two vanadium(V)-citrate complexes on the modulation of calcium accumulation and ATP hydrolysis by the SR calcium pump reflected the observed variable reactivity into the nature of key species forming upon dissolution of the title complexes in the reaction media.

Original languageEnglish
Pages (from-to)2355-2361
Number of pages7
JournalJournal of Inorganic Biochemistry
Volume99
Issue number12
DOIs
Publication statusPublished - 1 Dec 2005

Keywords

  • Calcium pump
  • Vanadium-citrate complex

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