Interactions among grapevine disease-causing fungi. The role of reactive oxygen species

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Botryosphaeria parva, Eutypa lata and Phomopsis viticola are ascomyceteous fungi responsible for severe canker and dieback in numerous woody plants. In grapevine, these pathogens colonise the wood mainly through pruning wounds, and the diseases gradually develop, leading to partial or total vine death. In the present study, the three fungal species were grown in Czapek Dox modified medium. Under these conditions, fungal colonies are able to distinguish self from non-self. The production of reactive oxygen species (ROS) was analysed by specifically staining for superoxide (O-2 center dot(-)) or peroxide (O-2(2-)) radicals. The presence of ROS in both isolated cultures and fungal interactions was confirmed. All fungi produced both radicals, in every interaction. However, the patterns of ROS production depend on the fungus itself and on the fungal species with which it is interacting, being also dependent on the presence of antioxidant compounds in the surrounding medium. It is as though a fungal species hierarchy could be established for every interaction under each set of conditions (i.e. habitat). The results obtained suggest that fungi display more complex behaviours than generally acknowledged. They are able to recognize potential contestants and built up defence reactions, as well as weaken plant defences and structures to induce infection.
Original languageUnknown
Pages (from-to)117-127
JournalPhytopathologia Mediterranea
Issue number1
Publication statusPublished - 1 Jan 2009

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