@article{b0c4d106d0d044eea0161d26591d16e6,
title = "Interaction of [VIVO(acac)2] with Human Serum Transferrin and Albumin",
abstract = "[VO(acac)2] is a remarkable vanadium compound and has potential as a therapeutic drug. It is important to clarify how it is transported in blood, but the reports addressing its binding to serum proteins have been contradictory. We use several spectroscopic and mass spectrometric techniques (ESI and MALDI-TOF), small-angle X-ray scattering and size exclusion chromatography (SEC) to characterize solutions containing [VO(acac)2] and either human serum apotransferrin (apoHTF) or albumin (HSA). DFT and modeling protein calculations are carried out to disclose the type of binding to apoHTF. The measured circular dichroism spectra, SEC and MALDI-TOF data clearly prove that at least two VO–acac moieties may bind to apoHTF, most probably forming [VIVO(acac)(apoHTF)] complexes with residues of the HTF binding sites. No indication of binding of [VO(acac)2] to HSA is obtained. We conclude that VIVO–acac species may be transported in blood by transferrin. At very low complex concentrations speciation calculations suggest that [(VO)(apoHTF)] species form.",
keywords = "acetylacetonate, circular dichroism, mass spectrometry, transferrin",
author = "Ielyzaveta Chorna and Isabel Cavaco and Somnath Roy and Kuznetsov, {Maxim L.} and N{\'a}dia Ribeiro and Gon{\c c}alo Justino and Fernanda Marques and Teresa Santos-Silva and Santos, {Marino F.A.} and Santos, {Hugo M.} and Capelo, {Jos{\'e} L.} and James Doutch and Pessoa, {Jo{\~a}o Costa}",
note = "sem pdf conforme despacho. Fundacao para a Ciencia e Tecnologia (FCT), Portugal (projects UID/Multi/04349/2013, UID/QUI/00100/2013, RECI/QEQ-QIN/0189/2012, RECI/QEQMED/0330/2012, PTDC/QEQ-MED/1902/2014 and doctoral grant SFRH/BD/77894/2011 to M.F.A.S) and programme FCT Investigator: IF/00100/2013 (IC) and IF/00007/2015 (H.M.S.). The PROTEOMASS Scientific Society is acknowledged by the funding provided to the Biological Mass Spectrometry Isabel Moura. H.M.S., J.L.C., M.F.A.S. and T.S.S acknowledge the funding provided by UCIBIO, Unidade de Ciencias Biomoleculares Aplicadas, (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). H.M.S. and J.L.C. also acknowledge the Associate Laboratory for Green Chemistry LAQV (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). The Portuguese NMR and Mass Spectrometry IST-UL Centers are also acknowledged for the access to the equipment. The authors would also like to thank the ESRF (Grenoble, France) for providing the access to the SAXS facilities (beamline BM29).{"}",
year = "2017",
month = aug,
day = "17",
doi = "10.1002/asia.201700469",
language = "English",
volume = "12",
pages = "2062--2084",
journal = "Chemistry - An Asian Journal",
issn = "1861-4728",
publisher = "WILEY-V C H VERLAG GMBH",
number = "16",
}