Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus

Robert Pjeta, Herbert Lindner, Leopold Kremser, Willi Salvenmoser, Daniel Sobral, Peter Ladurner, Romana Santos

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Echinoderms, such as the rock-boring sea urchin Paracentrotus lividus, attach temporarily to surfaces during locomotion using their tube feet. They can attach firmly to any substrate and release from it within seconds through the secretion of unknown molecules. The composition of the adhesive, as well as the releasing secretion, remains largely unknown. This study re-analyzed a differential proteome dataset from Lebesgue et al. by mapping mass spectrometry-derived peptides to a P. lividus de novo transcriptome generated in this study. This resulted in a drastic increase in mapped proteins in comparison to the previous publication. The data were subsequently combined with a differential RNAseq approach to identify potential adhesion candidate genes. A gene expression analysis of 59 transcripts using whole mount in situ hybridization led to the identification of 16 transcripts potentially involved in bioadhesion. In the future these data could be useful for the production of synthetic reversible adhesives for industrial and medical purposes.

Original languageEnglish
Article number946
JournalInternational Journal of Molecular Sciences
Volume21
Issue number3
DOIs
Publication statusPublished - Feb 2020

Keywords

  • Bioadhesion
  • Differential RNAseq
  • Echinoderm
  • In situ hybridization
  • Mass spectrometry
  • Sea urchin

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