TY - JOUR
T1 - Integration of HIV in the Human Genome
T2 - Which Sites Are Preferential? A Genetic and Statistical Assessment
AU - Gonçalves, Juliana
AU - Moreira, Elsa
AU - Sequeira, Inês Jorge da Silva
AU - Rodrigues, António S.
AU - Rueff, J.
AU - Brás, A.
N1 - WOS:000375972100001
PMID:27294106
PY - 2016
Y1 - 2016
N2 - Chromosomal fragile sites (FSs) are loci where gaps and breaks may occur and are preferential integration targets for some viruses, for example, Hepatitis B, Epstein-Barr virus, HPV16, HPV18, and MLV vectors. However, the integration of the human immunodeficiency virus (HIV) in Giemsa bands and in FSs is not yet completely clear. This study aimed to assess the integration preferences of HIV in FSs and in Giemsa bands using an in silico study. HIV integration positions from Jurkat cells were used and two nonparametric tests were applied to compare HIV integration in dark versus light bands and in FS versus non-FS (NFSs). The results show that light bands are preferential targets for integration of HIV-1 in Jurkat cells and also that it integrates with equal intensity in FSs and in NFSs. The data indicates that HIV displays different preferences for FSs compared to other viruses. The aim was to develop and apply an approach to predict the conditions and constraints of HIV insertion in the human genome which seems to adequately complement empirical data.
AB - Chromosomal fragile sites (FSs) are loci where gaps and breaks may occur and are preferential integration targets for some viruses, for example, Hepatitis B, Epstein-Barr virus, HPV16, HPV18, and MLV vectors. However, the integration of the human immunodeficiency virus (HIV) in Giemsa bands and in FSs is not yet completely clear. This study aimed to assess the integration preferences of HIV in FSs and in Giemsa bands using an in silico study. HIV integration positions from Jurkat cells were used and two nonparametric tests were applied to compare HIV integration in dark versus light bands and in FS versus non-FS (NFSs). The results show that light bands are preferential targets for integration of HIV-1 in Jurkat cells and also that it integrates with equal intensity in FSs and in NFSs. The data indicates that HIV displays different preferences for FSs compared to other viruses. The aim was to develop and apply an approach to predict the conditions and constraints of HIV insertion in the human genome which seems to adequately complement empirical data.
KW - chromosome fragile
KW - gene insertion
KW - Giemsa stain
KW - human cell
KW - human genome
KW - Human immunodeficiency virus 1
KW - jurkat cell line
KW - nonhuman
KW - nonparametric test
KW - virus DNA cell DNA interaction
UR - http://www.scopus.com/inward/record.url?scp=84971525140&partnerID=8YFLogxK
U2 - 10.1155/2016/2168590
DO - 10.1155/2016/2168590
M3 - Article
C2 - 27294106
AN - SCOPUS:84971525140
SN - 2314-436X
VL - 2016
JO - International Journal of Genomics
JF - International Journal of Genomics
M1 - 2168590
ER -
