Insect cells as production platform of complex virus-like particles

Fabiana Fernandes, Ana Palma Teixeira, Nuno Carinhas, Manuel José Teixeira Carrondo, Paula Maria Alves

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Virus-like particles (VLPs) are multiprotein structures that resemble the conformation of native viruses but lack a viral genome, potentiating their application as safer and cheaper vaccines. The production of VLPs has been strongly linked with the use of insect cells and the baculovirus expression vector system, especially those particles composed of two or more structural viral proteins. In fact, this expression platform has been extensively improved over the years to address the challenges of coexpression of multiple proteins and their proper assembly into complexes in the same cell. In this article, the role of insect cell technology in the development and production of complex VLPs is overviewed; recent achievements, current bottlenecks and future trends are also highlighted.
Original languageEnglish
Pages (from-to)225-236
JournalExpert Review of Vaccines
Volume12
Issue number2
DOIs
Publication statusPublished - 1 Jan 2013

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Virion
Insects
Viral Structural Proteins
Baculoviridae
Viral Genome
Vaccines
Viruses
Technology
Proteins

Cite this

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Insect cells as production platform of complex virus-like particles. / Fernandes, Fabiana; Teixeira, Ana Palma; Carinhas, Nuno; Carrondo, Manuel José Teixeira; Alves, Paula Maria.

In: Expert Review of Vaccines, Vol. 12, No. 2, 01.01.2013, p. 225-236.

Research output: Contribution to journalArticle

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AU - Fernandes, Fabiana

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AU - Alves, Paula Maria

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AB - Virus-like particles (VLPs) are multiprotein structures that resemble the conformation of native viruses but lack a viral genome, potentiating their application as safer and cheaper vaccines. The production of VLPs has been strongly linked with the use of insect cells and the baculovirus expression vector system, especially those particles composed of two or more structural viral proteins. In fact, this expression platform has been extensively improved over the years to address the challenges of coexpression of multiple proteins and their proper assembly into complexes in the same cell. In this article, the role of insect cell technology in the development and production of complex VLPs is overviewed; recent achievements, current bottlenecks and future trends are also highlighted.

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