TY - JOUR
T1 - Innovative Strategy for Aroma Stabilization Using Green Solvents
T2 - Supercritical CO2 Extracts of Satureja montana Dispersed in Deep Eutectic Solvents
AU - Vladić, Jelena
AU - Kovačević, Strahinja
AU - Aladić, Krunoslav
AU - Jokić, Stela
AU - Radman, Sanja
AU - Podunavac-Kuzmanović, Sanja
AU - Duarte, Ana Rita C.
AU - Jerković, Igor
N1 - Funding Information:
This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 101003396.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/7/14
Y1 - 2023/7/14
N2 - The aim of this work was to establish the potential of natural deep eutectic solvents (NADES) for the stabilization of aroma volatile organic compounds from a natural source. Satureja montana was used as a source of volatile components, as it is rich in terpenes of great commercial and biological importance, such as carvacrol, thymol, and thymoquinone, among others. Supercritical CO2 was used to extract the lipophilic fraction of S. montana, which was further directly dispersed in NADES. The stabilizing capacity of seven different NADES based on betaine and glycerol was analyzed. The stability of the components in NADES was monitored by analyzing the headspace profile during 6 months of storage at room temperature. The changes in the headspace profile over time were analyzed by using different statistical and chemometric tools and the Wilcoxon matched pair test. It was determined that alterations over time occurred such as degradation and oxidation, and they were the most prominent in the control. In addition, the indicator of decreased stability of the control was the formation of the new compounds that could compromise the quality of the product. In the stabilized NADES samples, the changes were significantly less prominent, indicating that the NADES had a stabilizing effect on the volatile compounds. According to Wilcoxon matched pair test, the most efficient stability was achieved by using betaine/ethylene glycol, glycerol/glucose, and betaine/sorbitol/water. Therefore, by applying two green solvents, a sustainable approach for obtaining pure and high-quality S. montana extracts with extended stability at room temperature was established.
AB - The aim of this work was to establish the potential of natural deep eutectic solvents (NADES) for the stabilization of aroma volatile organic compounds from a natural source. Satureja montana was used as a source of volatile components, as it is rich in terpenes of great commercial and biological importance, such as carvacrol, thymol, and thymoquinone, among others. Supercritical CO2 was used to extract the lipophilic fraction of S. montana, which was further directly dispersed in NADES. The stabilizing capacity of seven different NADES based on betaine and glycerol was analyzed. The stability of the components in NADES was monitored by analyzing the headspace profile during 6 months of storage at room temperature. The changes in the headspace profile over time were analyzed by using different statistical and chemometric tools and the Wilcoxon matched pair test. It was determined that alterations over time occurred such as degradation and oxidation, and they were the most prominent in the control. In addition, the indicator of decreased stability of the control was the formation of the new compounds that could compromise the quality of the product. In the stabilized NADES samples, the changes were significantly less prominent, indicating that the NADES had a stabilizing effect on the volatile compounds. According to Wilcoxon matched pair test, the most efficient stability was achieved by using betaine/ethylene glycol, glycerol/glucose, and betaine/sorbitol/water. Therefore, by applying two green solvents, a sustainable approach for obtaining pure and high-quality S. montana extracts with extended stability at room temperature was established.
KW - aromas
KW - deep eutectic solvent
KW - Satureja montana
KW - stabilization
KW - supercritical carbon dioxide
KW - volatile compounds
UR - http://www.scopus.com/inward/record.url?scp=85165975886&partnerID=8YFLogxK
U2 - 10.3390/biom13071126
DO - 10.3390/biom13071126
M3 - Article
C2 - 37509162
AN - SCOPUS:85165975886
SN - 2218-273X
VL - 13
JO - Biomolecules
JF - Biomolecules
IS - 7
M1 - 1126
ER -