Inhibition of Yap2 activity by MAPKAP kinase Rck1 affects yeast tolerance to cadmium

Daiane Mazzola, Catarina Pimentel, Soraia Caetano, Catarina Amaral, Regina Menezes, Claudia N. Santos, Elis Eleutherio, Claudina Amélia Pousada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Yap2 is a cadmium responsive transcription factor that interacts with MAPK-activated protein (MAPKAP) kinase Rck1. We show that Rck1 deletion confers protection against cadmium toxicity and that the mechanism underlying this observation relies on Yap2. Rck1 removal from the yeast genome potentiates Yap2 activity by increasing protein half-life and delaying its nuclear export. As a consequence, several Yap2 antioxidant targets are over-activated by a mechanism that also requires Yap1. Several genes of the cell wall integrity (CWI) pathway are upregulated under cadmium stress in a Yap2 dependent way. We showed that deletion of CWI genes renders yeast cells more sensitive to cadmium. These findings led us to suggest that in response to cadmium stress Yap2 may serve a dual purpose: oxidative stress attenuation and cell wall maintenance.

Original languageEnglish
Pages (from-to)2841-2849
Number of pages9
JournalFEBS Letters
Volume589
Issue number19
DOIs
Publication statusPublished - 24 Mar 2015

Keywords

  • Cadmium
  • MAPK-activated protein
  • Rck1
  • Yap2
  • Yeast

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