Influence of poly(N-isopropylacrylamide) and poly(N,N′-diethyl acrylamide) coatings on polysulfone/polyacrylonitrile-based membranes for protein separation

Telma Barroso; Raquel Viveiros; Margarida Coelho; Teresa Casimiro; Ana M. Botelho do Rego; Ana Aguiar-Ricardo

doi:10.1002/pat.2057

Influence of poly(N-isopropylacrylamide) and poly(N,N′-diethyl acrylamide) coatings on polysulfone/polyacrylonitrile-based membranes for protein separation

Telma Barroso, Raquel Viveiros, Margarida Coelho, Teresa Casimiro, Ana M. Botelho do Rego, Ana Aguiar-Ricardo

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Herein we describe the design and the assembly of temperature sensitive polysulfone (PS)/polyacrylonitrile (PAN) blend membranes using supercritical fluid technology. Blended membranes were prepared using the CO ₂-assisted phase inversion method, and their pores were coated with two thermoresponsive hydrogels-poly(N-isopropylacrylamide) (PNIPAAm) and poly(N,N′-diethylacrylamide) (PDEAAm). Permeation experiments of bovine serum albumin (BSA) and lysozyme (LYS) solutions were used to evaluate the performance and temperature-responsive behavior of coated membranes. While membranes coated with PNIPAAm presented similar protein permeation profiles at temperatures below and above its lower critical solution temperature, PDEAAm coating imparted a temperature-responsive behavior to PS/PAN (90:10) membranes and selective permeation of proteins with different sizes.

Original language	English
Pages (from-to)	1381-1393
Number of pages	13
Journal	Polymers For Advanced Technologies
Volume	23
Issue number	10
DOIs	https://doi.org/10.1002/pat.2057
Publication status	Published - 1 Oct 2012

Keywords

Hydrogels
Protein filtration
Supercritical carbon dioxide
Thermoresponsive membranes

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@article{1a58ee9225f74e68a21889320ca5ac10,

title = "Influence of poly(N-isopropylacrylamide) and poly(N,N′-diethyl acrylamide) coatings on polysulfone/polyacrylonitrile-based membranes for protein separation",

abstract = "Herein we describe the design and the assembly of temperature sensitive polysulfone (PS)/polyacrylonitrile (PAN) blend membranes using supercritical fluid technology. Blended membranes were prepared using the CO 2-assisted phase inversion method, and their pores were coated with two thermoresponsive hydrogels-poly(N-isopropylacrylamide) (PNIPAAm) and poly(N,N′-diethylacrylamide) (PDEAAm). Permeation experiments of bovine serum albumin (BSA) and lysozyme (LYS) solutions were used to evaluate the performance and temperature-responsive behavior of coated membranes. While membranes coated with PNIPAAm presented similar protein permeation profiles at temperatures below and above its lower critical solution temperature, PDEAAm coating imparted a temperature-responsive behavior to PS/PAN (90:10) membranes and selective permeation of proteins with different sizes.",

keywords = "Hydrogels, Protein filtration, Supercritical carbon dioxide, Thermoresponsive membranes",

author = "Telma Barroso and Raquel Viveiros and Margarida Coelho and Teresa Casimiro and {Botelho do Rego}, {Ana M.} and Ana Aguiar-Ricardo",

note = "The authors would like to thank the financial support from Fundacao para a Ciencia e Tecnologia (FCT-Lisbon) through contracts PTDC/CTM/70513/2006, MIT-Pt/BS-CTRM/0051/2008, doctoral grant SFRH/BD/62475/2009 (T. B.), doctoral grant SFRH/BD/75094/2010 (M. C.) and National NMR Network REDE/1517/RMN/2005, MIT-Portugal Program, Bioengineering Systems Focus Area, Fundacao Calouste Gulbenkian, FEDER and FSE. We wish to thank the Analytical Services Laboratory of REQUIMTE.",

year = "2012",

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doi = "10.1002/pat.2057",

language = "English",

volume = "23",

pages = "1381--1393",

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Influence of poly(N-isopropylacrylamide) and poly(N,N′-diethyl acrylamide) coatings on polysulfone/polyacrylonitrile-based membranes for protein separation. / Barroso, Telma; Viveiros, Raquel; Coelho, Margarida et al.
In: Polymers For Advanced Technologies, Vol. 23, No. 10, 01.10.2012, p. 1381-1393.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Influence of poly(N-isopropylacrylamide) and poly(N,N′-diethyl acrylamide) coatings on polysulfone/polyacrylonitrile-based membranes for protein separation

AU - Barroso, Telma

AU - Viveiros, Raquel

AU - Coelho, Margarida

AU - Casimiro, Teresa

AU - Botelho do Rego, Ana M.

AU - Aguiar-Ricardo, Ana

N1 - The authors would like to thank the financial support from Fundacao para a Ciencia e Tecnologia (FCT-Lisbon) through contracts PTDC/CTM/70513/2006, MIT-Pt/BS-CTRM/0051/2008, doctoral grant SFRH/BD/62475/2009 (T. B.), doctoral grant SFRH/BD/75094/2010 (M. C.) and National NMR Network REDE/1517/RMN/2005, MIT-Portugal Program, Bioengineering Systems Focus Area, Fundacao Calouste Gulbenkian, FEDER and FSE. We wish to thank the Analytical Services Laboratory of REQUIMTE.

PY - 2012/10/1

Y1 - 2012/10/1

N2 - Herein we describe the design and the assembly of temperature sensitive polysulfone (PS)/polyacrylonitrile (PAN) blend membranes using supercritical fluid technology. Blended membranes were prepared using the CO 2-assisted phase inversion method, and their pores were coated with two thermoresponsive hydrogels-poly(N-isopropylacrylamide) (PNIPAAm) and poly(N,N′-diethylacrylamide) (PDEAAm). Permeation experiments of bovine serum albumin (BSA) and lysozyme (LYS) solutions were used to evaluate the performance and temperature-responsive behavior of coated membranes. While membranes coated with PNIPAAm presented similar protein permeation profiles at temperatures below and above its lower critical solution temperature, PDEAAm coating imparted a temperature-responsive behavior to PS/PAN (90:10) membranes and selective permeation of proteins with different sizes.

AB - Herein we describe the design and the assembly of temperature sensitive polysulfone (PS)/polyacrylonitrile (PAN) blend membranes using supercritical fluid technology. Blended membranes were prepared using the CO 2-assisted phase inversion method, and their pores were coated with two thermoresponsive hydrogels-poly(N-isopropylacrylamide) (PNIPAAm) and poly(N,N′-diethylacrylamide) (PDEAAm). Permeation experiments of bovine serum albumin (BSA) and lysozyme (LYS) solutions were used to evaluate the performance and temperature-responsive behavior of coated membranes. While membranes coated with PNIPAAm presented similar protein permeation profiles at temperatures below and above its lower critical solution temperature, PDEAAm coating imparted a temperature-responsive behavior to PS/PAN (90:10) membranes and selective permeation of proteins with different sizes.

KW - Hydrogels

KW - Protein filtration

KW - Supercritical carbon dioxide

KW - Thermoresponsive membranes

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SP - 1381

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JO - Polymers For Advanced Technologies

JF - Polymers For Advanced Technologies

IS - 10

ER -

Influence of poly(N-isopropylacrylamide) and poly(N,N′-diethyl acrylamide) coatings on polysulfone/polyacrylonitrile-based membranes for protein separation

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Keywords

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