NMR spectroscopy was applied for quantitative and qualitative characterization of the chemical composition and microstructure of a series of poly(3-hydroxybutyrate-co-3-hydoxyvalerate) copolymers, P(3HB-co-3HV), synthesized by mixed microbial cultures at several different feeding strategies. The monomer sequence distribution of the bacterially synthesized P(3HB-co-3HV) was defined by analysis of their high-resolution 1D C-13 NMR and 2D H-1/C-13 HSQC and H-1/C-13 HMBC NMR spectra. The results were verified by employment of statistical methods and suggest a block copolymer microstructure of the P(3HB-co-3HV) copolymers studied. Definitive distinction between block copolymers or a mixture of random copolymers could not be achieved. NMR spectral analysis indicates that the chemical composition and microstructure of the copolymers can be tuned by choosing a correct feeding strategy. Copyright (C) 2009 John Wiley & Sons, Ltd.
|Journal||Magnetic Resonance in Chemistry|
|Publication status||Published - 1 Jan 2009|
Lemos, P. A. D. C., Reis, M. D. C. F. M., Cabrita, E. J. D. S., & Ramos, A. M. M. (2009). Influence of feeding strategies of mixed microbial cultures on the chemical composition and microstructure of copolyesters P(3HB-co-3HV) analyzed by NMR and statistical analysis. Magnetic Resonance in Chemistry, 47(6), 497-504.