Influence of feeding strategies of mixed microbial cultures on the chemical composition and microstructure of copolyesters P(3HB-co-3HV) analyzed by NMR and statistical analysis

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Abstract

NMR spectroscopy was applied for quantitative and qualitative characterization of the chemical composition and microstructure of a series of poly(3-hydroxybutyrate-co-3-hydoxyvalerate) copolymers, P(3HB-co-3HV), synthesized by mixed microbial cultures at several different feeding strategies. The monomer sequence distribution of the bacterially synthesized P(3HB-co-3HV) was defined by analysis of their high-resolution 1D C-13 NMR and 2D H-1/C-13 HSQC and H-1/C-13 HMBC NMR spectra. The results were verified by employment of statistical methods and suggest a block copolymer microstructure of the P(3HB-co-3HV) copolymers studied. Definitive distinction between block copolymers or a mixture of random copolymers could not be achieved. NMR spectral analysis indicates that the chemical composition and microstructure of the copolymers can be tuned by choosing a correct feeding strategy. Copyright (C) 2009 John Wiley & Sons, Ltd.
Original languageUnknown
Pages (from-to)497-504
JournalMagnetic Resonance in Chemistry
Volume47
Issue number6
Publication statusPublished - 1 Jan 2009

Cite this

@article{b09d252626c44c2ca7df0de5db6ec5f2,
title = "Influence of feeding strategies of mixed microbial cultures on the chemical composition and microstructure of copolyesters P(3HB-co-3HV) analyzed by NMR and statistical analysis",
abstract = "NMR spectroscopy was applied for quantitative and qualitative characterization of the chemical composition and microstructure of a series of poly(3-hydroxybutyrate-co-3-hydoxyvalerate) copolymers, P(3HB-co-3HV), synthesized by mixed microbial cultures at several different feeding strategies. The monomer sequence distribution of the bacterially synthesized P(3HB-co-3HV) was defined by analysis of their high-resolution 1D C-13 NMR and 2D H-1/C-13 HSQC and H-1/C-13 HMBC NMR spectra. The results were verified by employment of statistical methods and suggest a block copolymer microstructure of the P(3HB-co-3HV) copolymers studied. Definitive distinction between block copolymers or a mixture of random copolymers could not be achieved. NMR spectral analysis indicates that the chemical composition and microstructure of the copolymers can be tuned by choosing a correct feeding strategy. Copyright (C) 2009 John Wiley & Sons, Ltd.",
keywords = "copolymers, statistical, poly(hydroxyalkanoates), microstructure, poly(beta-hydroxybutyrate), copolyesters, microbial, analysis, H-1, nuclear-magnetic-resonance, NMR, C-13, bacterial, chemical, composition, poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate), polymers, polyhydroxyalkanoates, poly(3-hydroxybutyrate-co-3-hydroxyvalerate)",
author = "Lemos, {Paulo Alexandre da Costa} and Reis, {Maria D'ascens{\~a}o Carvalho Fernandes Miranda} and Cabrita, {Eurico Jos{\'e} da Silva} and Ramos, {Ana Maria Martelo}",
year = "2009",
month = "1",
day = "1",
language = "Unknown",
volume = "47",
pages = "497--504",
journal = "Magnetic Resonance In Chemistry",
issn = "0749-1581",
publisher = "John Wiley & Sons, Ltd",
number = "6",

}

TY - JOUR

T1 - Influence of feeding strategies of mixed microbial cultures on the chemical composition and microstructure of copolyesters P(3HB-co-3HV) analyzed by NMR and statistical analysis

AU - Lemos, Paulo Alexandre da Costa

AU - Reis, Maria D'ascensão Carvalho Fernandes Miranda

AU - Cabrita, Eurico José da Silva

AU - Ramos, Ana Maria Martelo

PY - 2009/1/1

Y1 - 2009/1/1

N2 - NMR spectroscopy was applied for quantitative and qualitative characterization of the chemical composition and microstructure of a series of poly(3-hydroxybutyrate-co-3-hydoxyvalerate) copolymers, P(3HB-co-3HV), synthesized by mixed microbial cultures at several different feeding strategies. The monomer sequence distribution of the bacterially synthesized P(3HB-co-3HV) was defined by analysis of their high-resolution 1D C-13 NMR and 2D H-1/C-13 HSQC and H-1/C-13 HMBC NMR spectra. The results were verified by employment of statistical methods and suggest a block copolymer microstructure of the P(3HB-co-3HV) copolymers studied. Definitive distinction between block copolymers or a mixture of random copolymers could not be achieved. NMR spectral analysis indicates that the chemical composition and microstructure of the copolymers can be tuned by choosing a correct feeding strategy. Copyright (C) 2009 John Wiley & Sons, Ltd.

AB - NMR spectroscopy was applied for quantitative and qualitative characterization of the chemical composition and microstructure of a series of poly(3-hydroxybutyrate-co-3-hydoxyvalerate) copolymers, P(3HB-co-3HV), synthesized by mixed microbial cultures at several different feeding strategies. The monomer sequence distribution of the bacterially synthesized P(3HB-co-3HV) was defined by analysis of their high-resolution 1D C-13 NMR and 2D H-1/C-13 HSQC and H-1/C-13 HMBC NMR spectra. The results were verified by employment of statistical methods and suggest a block copolymer microstructure of the P(3HB-co-3HV) copolymers studied. Definitive distinction between block copolymers or a mixture of random copolymers could not be achieved. NMR spectral analysis indicates that the chemical composition and microstructure of the copolymers can be tuned by choosing a correct feeding strategy. Copyright (C) 2009 John Wiley & Sons, Ltd.

KW - copolymers

KW - statistical

KW - poly(hydroxyalkanoates)

KW - microstructure

KW - poly(beta-hydroxybutyrate)

KW - copolyesters

KW - microbial

KW - analysis

KW - H-1

KW - nuclear-magnetic-resonance

KW - NMR

KW - C-13

KW - bacterial

KW - chemical

KW - composition

KW - poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate)

KW - polymers

KW - polyhydroxyalkanoates

KW - poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

M3 - Article

VL - 47

SP - 497

EP - 504

JO - Magnetic Resonance In Chemistry

JF - Magnetic Resonance In Chemistry

SN - 0749-1581

IS - 6

ER -