Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes

Miguel Viveiros; Ana R. Jesus; Mafalda Brito; Clara Leandro; Marta Martins; Diane Ordway; Ana Maria Molnar; Joseph Molnar; Leonard Amaral

doi:10.1128/AAC.49.8.3578-3582.2005

Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes

Miguel Viveiros, Ana R. Jesus, Mafalda Brito, Clara Leandro, Marta Martins, Diane Ordway, Ana Maria Molnar, Joseph Molnar, Leonard Amaral

Research output: Contribution to journal › Article › peer-review

122 Citations (Scopus)

Abstract

Expression of eight transporter genes of Escherichia coli K-12 and its ΔacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the ΔacrAB tetracycline-induced strain.

Original language	English
Pages (from-to)	3578-3582
Number of pages	5
Journal	Antimicrobial Agents and Chemotherapy
Volume	49
Issue number	8
DOIs	https://doi.org/10.1128/AAC.49.8.3578-3582.2005
Publication status	Published - Aug 2005

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10.1128/AAC.49.8.3578-3582.2005

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Viveiros, M., Jesus, A. R., Brito, M., Leandro, C., Martins, M., Ordway, D., Molnar, A. M., Molnar, J., & Amaral, L. (2005). Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes. Antimicrobial Agents and Chemotherapy, 49(8), 3578-3582. https://doi.org/10.1128/AAC.49.8.3578-3582.2005

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abstract = "Expression of eight transporter genes of Escherichia coli K-12 and its ΔacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the ΔacrAB tetracycline-induced strain.",

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Viveiros, M, Jesus, AR, Brito, M, Leandro, C, Martins, M, Ordway, D, Molnar, AM, Molnar, J & Amaral, L 2005, 'Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes', Antimicrobial Agents and Chemotherapy, vol. 49, no. 8, pp. 3578-3582. https://doi.org/10.1128/AAC.49.8.3578-3582.2005

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T1 - Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes

AU - Viveiros, Miguel

AU - Jesus, Ana R.

AU - Brito, Mafalda

AU - Leandro, Clara

AU - Martins, Marta

AU - Ordway, Diane

AU - Molnar, Ana Maria

AU - Molnar, Joseph

AU - Amaral, Leonard

PY - 2005/8

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N2 - Expression of eight transporter genes of Escherichia coli K-12 and its ΔacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the ΔacrAB tetracycline-induced strain.

AB - Expression of eight transporter genes of Escherichia coli K-12 and its ΔacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the ΔacrAB tetracycline-induced strain.

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ER -

Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes

Abstract

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