TY - JOUR
T1 - Inactivation of Aspergillus species in real water matrices using medium pressure mercury lamps
AU - Oliveira, B. R.
AU - Marques, A. P.
AU - Ressurreição, Mariana
AU - Moreira, Carlos Jorge da Silva
AU - S.Pereira, Cristina Isabel Tavares
AU - B. Crespo, M. T.
AU - Pereira, V. J.
N1 - Funding Information:
The authors would like to thank Dr. David Bastien from SYSMEX for helping with the flow cytometry analysis, In?s Lu?s with protein quantification as well as Bruno Alexandre, Ricardo Gomes and Muhammad Asif for fruitful discussions. Mass spectrometry data were generated by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal. The authors also thank SYSMEX that has kindly provided the flow cytometer used in these experiments. Financial support from Funda??o para a Ci?ncia e a Tecnologia through the fellowship SFRH/BD/111150/2015 and project PTDC/EAM-AMB/30989/2017 is gratefully acknowledged. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Funda??o para a Ci?ncia e Tecnologia/Minist?rio da Educa??o e Ci?ncia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is gratefully acknowledged. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged.
Funding Information:
The authors would like to thank Dr. David Bastien from SYSMEX for helping with the flow cytometry analysis, Inês Luís with protein quantification as well as Bruno Alexandre, Ricardo Gomes and Muhammad Asif for fruitful discussions. Mass spectrometry data were generated by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal. The authors also thank SYSMEX that has kindly provided the flow cytometer used in these experiments. Financial support from Fundação para a Ciência e a Tecnologia through the fellowship SFRH/BD/111150/2015 and project PTDC/EAM-AMB/30989/2017 is gratefully acknowledged. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência , through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is gratefully acknowledged. Funding from INTERFACE Programme , through the Innovation, Technology and Circular Economy Fund (FITEC) , is gratefully acknowledged.
Publisher Copyright:
© 2021
PY - 2021/8
Y1 - 2021/8
N2 - The aim of this work is to understand the inactivation efficiency of medium pressure mercury lamps, measured in terms of growth inhibition as well as cell death, damage and response, using three strains from three different Aspergillus species (A. fumigatus, A. niger and, A. terreus) spiked in filtered surface water. A complete characterization of the effect of the treatment on each strain of the fungal species was assessed considering spores' morphology, cell wall integrity and enzymatic activity, the formation of pyrimidine dimers in the DNA and proteome analysis. Results showed that, when subjected to medium pressure mercury lamps, A. niger is the most resistant to inactivation, that both A. fumigatus and A. niger suffer more morphological changes and present a higher number of damaged spores and A. terreus presented more dead spores. DNA damages detected in A. niger were able to be repaired to some extent, under both light and dark conditions. Finally, proteome analysis showed that the UV radiation treatment triggered different types of stress response, including cell wall reorganization and DNA repair in A. fumigatus and A. terreus, and oxidative stress responses like the increase in production of citric acid and itaconic acid in A. niger and A. terreus, respectively.
AB - The aim of this work is to understand the inactivation efficiency of medium pressure mercury lamps, measured in terms of growth inhibition as well as cell death, damage and response, using three strains from three different Aspergillus species (A. fumigatus, A. niger and, A. terreus) spiked in filtered surface water. A complete characterization of the effect of the treatment on each strain of the fungal species was assessed considering spores' morphology, cell wall integrity and enzymatic activity, the formation of pyrimidine dimers in the DNA and proteome analysis. Results showed that, when subjected to medium pressure mercury lamps, A. niger is the most resistant to inactivation, that both A. fumigatus and A. niger suffer more morphological changes and present a higher number of damaged spores and A. terreus presented more dead spores. DNA damages detected in A. niger were able to be repaired to some extent, under both light and dark conditions. Finally, proteome analysis showed that the UV radiation treatment triggered different types of stress response, including cell wall reorganization and DNA repair in A. fumigatus and A. terreus, and oxidative stress responses like the increase in production of citric acid and itaconic acid in A. niger and A. terreus, respectively.
KW - Aspergillus
KW - Cellular response
KW - Medium pressure mercury lamps
KW - Reactivation
KW - Water disinfection
UR - http://www.scopus.com/inward/record.url?scp=85108887608&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2021.112242
DO - 10.1016/j.jphotobiol.2021.112242
M3 - Article
C2 - 34214837
AN - SCOPUS:85108887608
SN - 1011-1344
VL - 221
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
M1 - 112242
ER -