Abstract

The physiological roles of insulin and nitric oxide (NO) have recently been recognized by several studies. A diversity of chemical modifications of insulin is reported both in vivo and in vitro. S-nitrosation, the covalent linkage of NO to cysteine-free thiol, is recognized as an important post-translational regulation in many proteins. Here, we report the in vitro synthesis of a S-nitrosothiol of bovine insulin A- and B-chains. These compounds were characterized by their HPLC chromatographic behavior, monitored by UV visible spectroscopy and electron spray ionization mass spectrometry. The experimental results indicate that each A- and B-chain were S-nitrosated with only one NO group. Stability and solubility of these synthesized derivatives is described for physiological purposes. In this work, nitroso A- and B-chains of insulin were synthesized in vitro in order to better understand the possible interactions between insulin and NO that may be involved in the etiology of insulin resistance.
Original languageEnglish
Pages (from-to)331-338
JournalEuropean Journal Of Mass Spectrometry
Volume12
Issue number5
DOIs
Publication statusPublished - 2006

Keywords

  • A-chain
  • B-chain
  • Insulin
  • Nitric oxide (NO)
  • Nitrosothiol

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