TY - JOUR
T1 - In vitro evaluation of alkaline lignins as antiparasitic agents and their use as an excipient in the release of benznidazole
AU - da Cruz Filho, Iranildo José
AU - Duarte, Denise Maria Figueiredo Araujo
AU - da Conceição Alves de Lima, Douglas
AU - Marques, Diego Santa Clara
AU - Dos Santos, Fábio André Brayner
AU - Alves, Luiz Carlos
AU - de Lima Aires, André
AU - Nogueira, Fátima
AU - do Carmo Alves de Lima, Maria
N1 - Copyright © 2023. Published by Elsevier B.V.
PY - 2023/3/15
Y1 - 2023/3/15
N2 - The Amazon rainforest is considered the largest tropical timber reserve in the world. The management of native forests in the Amazon is one of the most sensitive geopolitical issues today, given its national and international dimension. In this work, we obtained and characterized physicochemical lignins extracted from branches and leaves of Protium puncticulatum and Scleronema micranthum. In addition, we evaluated in vitro its potential as an antioxidant, cytotoxic agent against animal cells and antiparasitic against promastigotes of Leishmania amazonensis, trypomastigotes of T. cruzi and against Plasmodium falciparum parasites sensitive and resistant to chloroquine. The results showed that the lignins obtained are of the GSH type and have higher levels of guaiacyl units. However, they show structural differences as shown by spectroscopic analysis and radar charts. As for biological activities, they showed antioxidant potential and low cytotoxicity against animal cells. Antileishmanial/trypanocidal assays have shown that lignins can inhibit the growth of promastigotes and trypomastigotes in vitro. The lignins in this study showed low anti-Plasmodium falciparum activity against susceptible strains of Plasmodium falciparum and were able to inhibit the growth of the chloroquine-resistant strain. And were not able to inhibit the growth of Schistosoma mansoni parasites. Finally, lignins proved to be promising excipients in the release of benznidazole. These findings show the potential of these lignins not yet studied to promote different biological activities.
AB - The Amazon rainforest is considered the largest tropical timber reserve in the world. The management of native forests in the Amazon is one of the most sensitive geopolitical issues today, given its national and international dimension. In this work, we obtained and characterized physicochemical lignins extracted from branches and leaves of Protium puncticulatum and Scleronema micranthum. In addition, we evaluated in vitro its potential as an antioxidant, cytotoxic agent against animal cells and antiparasitic against promastigotes of Leishmania amazonensis, trypomastigotes of T. cruzi and against Plasmodium falciparum parasites sensitive and resistant to chloroquine. The results showed that the lignins obtained are of the GSH type and have higher levels of guaiacyl units. However, they show structural differences as shown by spectroscopic analysis and radar charts. As for biological activities, they showed antioxidant potential and low cytotoxicity against animal cells. Antileishmanial/trypanocidal assays have shown that lignins can inhibit the growth of promastigotes and trypomastigotes in vitro. The lignins in this study showed low anti-Plasmodium falciparum activity against susceptible strains of Plasmodium falciparum and were able to inhibit the growth of the chloroquine-resistant strain. And were not able to inhibit the growth of Schistosoma mansoni parasites. Finally, lignins proved to be promising excipients in the release of benznidazole. These findings show the potential of these lignins not yet studied to promote different biological activities.
KW - Animals
KW - Antiparasitic Agents/therapeutic use
KW - Lignin/therapeutic use
KW - Excipients
KW - Antioxidants/therapeutic use
KW - Trypanosoma cruzi
KW - Chagas Disease/drug therapy
KW - Chloroquine
U2 - 10.1016/j.ijbiomac.2023.123339
DO - 10.1016/j.ijbiomac.2023.123339
M3 - Article
C2 - 36682648
SN - 0141-8130
VL - 231
SP - 123339
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 123339
ER -