TY - JOUR
T1 - Improving succinic acid production by Actinobacillus succinogenes from raw industrial carob pods
AU - Carvalho, Margarida
AU - Roca, Christophe
AU - Reis, Maria A.M.
N1 - sem pdf conforme despacho.
Fundação para a Ciência e Tecnologia for the Ph.D. fellowship SFRH/BD/75032/2010.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Carob pods are an inexpensive by-product of locust bean gum industry that can be used as renewable feedstock for bio-based succinic acid. Here, for the first time, unprocessed raw carob pods were used to extract a highly enriched sugar solution, afterwards used as substrate to produce succinic acid using Actinobacillus succinogenes. Batch fermentations containing 30 g/L sugars resulted in a production rate of 1.67 g SA/L.h and a yield of 0.39 g SA/g sugars. Taking advantage of A. succinogenes’ metabolism, uncoupling cell growth from succinic acid production, a fed-batch mode was implemented to increase succinic acid yield and reduce by-products formation. This strategy resulted in a succinic acid yield of 0.94 g SA/g sugars, the highest yield reported in the literature for fed-batch and continuous experiments, while maintaining by-products at residual values. Results demonstrate that raw carob pods are a highly efficient feedstock for bio-based succinic acid production.
AB - Carob pods are an inexpensive by-product of locust bean gum industry that can be used as renewable feedstock for bio-based succinic acid. Here, for the first time, unprocessed raw carob pods were used to extract a highly enriched sugar solution, afterwards used as substrate to produce succinic acid using Actinobacillus succinogenes. Batch fermentations containing 30 g/L sugars resulted in a production rate of 1.67 g SA/L.h and a yield of 0.39 g SA/g sugars. Taking advantage of A. succinogenes’ metabolism, uncoupling cell growth from succinic acid production, a fed-batch mode was implemented to increase succinic acid yield and reduce by-products formation. This strategy resulted in a succinic acid yield of 0.94 g SA/g sugars, the highest yield reported in the literature for fed-batch and continuous experiments, while maintaining by-products at residual values. Results demonstrate that raw carob pods are a highly efficient feedstock for bio-based succinic acid production.
KW - Actinobacillus succinogenes
KW - Carob pods
KW - Fed-batch fermentation
KW - Succinic acid
UR - http://www.scopus.com/inward/record.url?scp=84977610865&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.06.140
DO - 10.1016/j.biortech.2016.06.140
M3 - Article
C2 - 27394995
AN - SCOPUS:84977610865
SN - 0960-8524
VL - 218
SP - 491
EP - 497
JO - Bioresource Technology
JF - Bioresource Technology
ER -