Improving polyhydroxyalkanoates storage capacity of a mixed microbial culture fed with hardwood sulfite spent liquor

Diogo Queirós, Alexandre Fonseca, Paulo Alexandre da Costa Lemos, Luísa S. Serafim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

BACKGROUND: The development of sustainable industrial processes demands the recovery and transformation of their by-products. The production of polyhydroxyalkanoates (PHAs) by mixed microbial cultures (MMC) is one of the available routes. This study evaluated the possibility of applying a two-step PHAs production process using an industrial by-product, hardwood sulphite spent liquor (HSSL), as feedstock for a MMC. The first step consisted in the selection of PHAs-storing microorganisms using an aerobic dynamic feeding strategy followed by a second step, accumulation. RESULTS: The selected MMC, despite not being able to use the main carbon source of HSSL, lignosulphonates, revealed the capacity to consume acetate and xylose to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and glucose biopolymer. The maximum PHAs content was relatively low, 6.6%, with a maximum production yield of 0.49 C-mmol HA C-mmol S−1 during the selection stage. CONCLUSION: A MMC was able to adapt to HSSL by consuming acetate and xylose. The imposed operational conditions resulted in a partial selection of the culture, since only acetate-consuming organisms experienced a real feast/famine regime, resulting in low PHAs production. © 2015 Society of Chemical Industry.
Original languageEnglish
Pages (from-to)2480-2487
JournalJournal of Chemical Technology and Biotechnology
Volume91
Issue number9
DOIs
Publication statusPublished - Sep 2016

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Hardwoods
Xylose
Byproducts
Biopolymers
Microorganisms
Feedstocks
Glucose
Recovery
Carbon

Keywords

  • aerobic process
  • bioconversion
  • kinetic parameters
  • mixed microbial cultures
  • polyhydroxyalkanoates
  • waste treatment

Cite this

@article{a4f5f107f3564737959c5e414009c502,
title = "Improving polyhydroxyalkanoates storage capacity of a mixed microbial culture fed with hardwood sulfite spent liquor",
abstract = "BACKGROUND: The development of sustainable industrial processes demands the recovery and transformation of their by-products. The production of polyhydroxyalkanoates (PHAs) by mixed microbial cultures (MMC) is one of the available routes. This study evaluated the possibility of applying a two-step PHAs production process using an industrial by-product, hardwood sulphite spent liquor (HSSL), as feedstock for a MMC. The first step consisted in the selection of PHAs-storing microorganisms using an aerobic dynamic feeding strategy followed by a second step, accumulation. RESULTS: The selected MMC, despite not being able to use the main carbon source of HSSL, lignosulphonates, revealed the capacity to consume acetate and xylose to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and glucose biopolymer. The maximum PHAs content was relatively low, 6.6{\%}, with a maximum production yield of 0.49 C-mmol HA C-mmol S−1 during the selection stage. CONCLUSION: A MMC was able to adapt to HSSL by consuming acetate and xylose. The imposed operational conditions resulted in a partial selection of the culture, since only acetate-consuming organisms experienced a real feast/famine regime, resulting in low PHAs production. {\circledC} 2015 Society of Chemical Industry.",
keywords = "aerobic process , bioconversion , kinetic parameters , mixed microbial cultures , polyhydroxyalkanoates , waste treatment",
author = "Diogo Queir{\'o}s and Alexandre Fonseca and Lemos, {Paulo Alexandre da Costa} and Serafim, {Lu{\'i}sa S.}",
note = "Sem PDF. project CICECO-Aveiro Institute of Materials (FCT UID/CTM/50011/2013); national funds through the FCT/MEC; FEDER; FCT (SFRH/BD/87758/2012)",
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Improving polyhydroxyalkanoates storage capacity of a mixed microbial culture fed with hardwood sulfite spent liquor. / Queirós, Diogo; Fonseca, Alexandre; Lemos, Paulo Alexandre da Costa; Serafim, Luísa S.

In: Journal of Chemical Technology and Biotechnology, Vol. 91, No. 9, 09.2016, p. 2480-2487.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Improving polyhydroxyalkanoates storage capacity of a mixed microbial culture fed with hardwood sulfite spent liquor

AU - Queirós, Diogo

AU - Fonseca, Alexandre

AU - Lemos, Paulo Alexandre da Costa

AU - Serafim, Luísa S.

N1 - Sem PDF. project CICECO-Aveiro Institute of Materials (FCT UID/CTM/50011/2013); national funds through the FCT/MEC; FEDER; FCT (SFRH/BD/87758/2012)

PY - 2016/9

Y1 - 2016/9

N2 - BACKGROUND: The development of sustainable industrial processes demands the recovery and transformation of their by-products. The production of polyhydroxyalkanoates (PHAs) by mixed microbial cultures (MMC) is one of the available routes. This study evaluated the possibility of applying a two-step PHAs production process using an industrial by-product, hardwood sulphite spent liquor (HSSL), as feedstock for a MMC. The first step consisted in the selection of PHAs-storing microorganisms using an aerobic dynamic feeding strategy followed by a second step, accumulation. RESULTS: The selected MMC, despite not being able to use the main carbon source of HSSL, lignosulphonates, revealed the capacity to consume acetate and xylose to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and glucose biopolymer. The maximum PHAs content was relatively low, 6.6%, with a maximum production yield of 0.49 C-mmol HA C-mmol S−1 during the selection stage. CONCLUSION: A MMC was able to adapt to HSSL by consuming acetate and xylose. The imposed operational conditions resulted in a partial selection of the culture, since only acetate-consuming organisms experienced a real feast/famine regime, resulting in low PHAs production. © 2015 Society of Chemical Industry.

AB - BACKGROUND: The development of sustainable industrial processes demands the recovery and transformation of their by-products. The production of polyhydroxyalkanoates (PHAs) by mixed microbial cultures (MMC) is one of the available routes. This study evaluated the possibility of applying a two-step PHAs production process using an industrial by-product, hardwood sulphite spent liquor (HSSL), as feedstock for a MMC. The first step consisted in the selection of PHAs-storing microorganisms using an aerobic dynamic feeding strategy followed by a second step, accumulation. RESULTS: The selected MMC, despite not being able to use the main carbon source of HSSL, lignosulphonates, revealed the capacity to consume acetate and xylose to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and glucose biopolymer. The maximum PHAs content was relatively low, 6.6%, with a maximum production yield of 0.49 C-mmol HA C-mmol S−1 during the selection stage. CONCLUSION: A MMC was able to adapt to HSSL by consuming acetate and xylose. The imposed operational conditions resulted in a partial selection of the culture, since only acetate-consuming organisms experienced a real feast/famine regime, resulting in low PHAs production. © 2015 Society of Chemical Industry.

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