Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions

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Abstract

Polyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30 CmM) and specific light intensities around 20 W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83 ± 0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21 ± 0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in <4 h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20 W/gX are sufficient to drive phototrophic PHA production processes.

Original languageEnglish
Pages (from-to)1085-1092
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume126
DOIs
Publication statusPublished - 1 Apr 2019

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Polyhydroxyalkanoates
Light
Acetates
Cheeses
Sunlight
Cheese
Electron energy levels
Nitrogen
Carbon
Substrates

Keywords

  • Fermented cheese whey (FCW)
  • Phototrophic mixed cultures (PMCs)
  • Polyhydroxyalkanoates (PHA)
  • Purple phototrophic bacteria (PPB)
  • Volatile fatty acids (VFAs)

Cite this

@article{0e1b312f04b44fee8b6f870009208efc,
title = "Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions",
abstract = "Polyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30 CmM) and specific light intensities around 20 W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83 ± 0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21 ± 0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30{\%} in <4 h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12{\%}. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20 W/gX are sufficient to drive phototrophic PHA production processes.",
keywords = "Fermented cheese whey (FCW), Phototrophic mixed cultures (PMCs), Polyhydroxyalkanoates (PHA), Purple phototrophic bacteria (PPB), Volatile fatty acids (VFAs)",
author = "Fradinho, {J. C.} and A. Oehmen and Reis, {M. A. M.}",
note = "The authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. Applied Molecular Biosciences Unit -UCIBIO acknowledges financing by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). NoAW project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement no 688338).",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.ijbiomac.2018.12.270",
language = "English",
volume = "126",
pages = "1085--1092",
journal = "International Journal of Biological Macromolecules",
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publisher = "Elsevier Science B.V., Inc",

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TY - JOUR

T1 - Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions

AU - Fradinho, J. C.

AU - Oehmen, A.

AU - Reis, M. A. M.

N1 - The authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. Applied Molecular Biosciences Unit -UCIBIO acknowledges financing by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). NoAW project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement no 688338).

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Polyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30 CmM) and specific light intensities around 20 W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83 ± 0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21 ± 0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in <4 h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20 W/gX are sufficient to drive phototrophic PHA production processes.

AB - Polyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30 CmM) and specific light intensities around 20 W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83 ± 0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21 ± 0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in <4 h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20 W/gX are sufficient to drive phototrophic PHA production processes.

KW - Fermented cheese whey (FCW)

KW - Phototrophic mixed cultures (PMCs)

KW - Polyhydroxyalkanoates (PHA)

KW - Purple phototrophic bacteria (PPB)

KW - Volatile fatty acids (VFAs)

UR - http://www.scopus.com/inward/record.url?scp=85059552216&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2018.12.270

DO - 10.1016/j.ijbiomac.2018.12.270

M3 - Article

VL - 126

SP - 1085

EP - 1092

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -