Improved virus purification processes for vaccines and gene therapy

Piergiuseppe Nestola; Cristina Peixoto; Ricardo R J S Silva; Paula M. Alves; José P B Mota; Manuel J T Carrondo

doi:10.1002/bit.25545

Improved virus purification processes for vaccines and gene therapy

Piergiuseppe Nestola, Cristina Peixoto, Ricardo R J S Silva, Paula M. Alves, José P B Mota, Manuel J T Carrondo

Research output: Contribution to journal › Review article › peer-review

111 Citations (Scopus)

Abstract

The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral downstream purification processes, encompassing the classical unit operations and their recent developments. Emphasis is given to novel strategies for process intensification, such as continuous or semi-continuous systems based on multicolumn technology, opening up process efficiency. Process understanding in the light of the pharmaceutical quality by design (QbD) initiative is also discussed. Finally, an outlook of the upcoming breakthrough technologies is presented. Biotechnol. Bioeng. 2015;112: 843-857.

Original language	English
Pages (from-to)	843-857
Number of pages	15
Journal	Biotechnology and Bioengineering
Volume	112
Issue number	5
DOIs	https://doi.org/10.1002/bit.25545
Publication status	Published - 1 May 2015

Keywords

Continuous purification
Downstream bioprocessing
Gene therapy
Process intensification
Vaccines

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/bit.25545

Cite this

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AU - Nestola, Piergiuseppe

AU - Peixoto, Cristina

AU - Silva, Ricardo R J S

AU - Alves, Paula M.

AU - Mota, José P B

AU - Carrondo, Manuel J T

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KW - Continuous purification

KW - Downstream bioprocessing

KW - Gene therapy

KW - Process intensification

KW - Vaccines

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Improved virus purification processes for vaccines and gene therapy

Abstract

Keywords

UN SDGs

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