Improved virus purification processes for vaccines and gene therapy

Piergiuseppe Nestola, Cristina Peixoto, Ricardo R J S Silva, Paula M. Alves, José P B Mota, Manuel J T Carrondo

Research output: Contribution to journalReview articlepeer-review

105 Citations (Scopus)


The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral downstream purification processes, encompassing the classical unit operations and their recent developments. Emphasis is given to novel strategies for process intensification, such as continuous or semi-continuous systems based on multicolumn technology, opening up process efficiency. Process understanding in the light of the pharmaceutical quality by design (QbD) initiative is also discussed. Finally, an outlook of the upcoming breakthrough technologies is presented. Biotechnol. Bioeng. 2015;112: 843-857.

Original languageEnglish
Pages (from-to)843-857
Number of pages15
JournalBiotechnology and Bioengineering
Issue number5
Publication statusPublished - 1 May 2015


  • Continuous purification
  • Downstream bioprocessing
  • Gene therapy
  • Process intensification
  • Vaccines


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