Abstract
Axillary bud multiplication and direct adventitious bud regeneration were improved for clonal propagation of Portuguese genotypes of Pinus pinaster. Axillary buds induced in an ex vitro two year-old genotype by 6-benzylaminopurine (BAP) spraying and apex removal were used for the establishment of in vitro axillary bud cultures. Improved procedures were also developed for both axillary bud multiplication from four week-old seedlings and direct adventitious bud induction from cotyledons. The first in vitro multiplication cycle of buds derived from the ex vitro material resulted in an approximately five-fold multiplication rate. Regarding the axillary buds derived from four-week-old seedlings, the best induction medium for bud multiplication was based on a GD medium plus 1.11 μM BAP and 0.27 μM α- naphthaleneacetic acid (NAA). Induction of adventitious buds varied significantly among seed provenances. In the best performing provenance, the highest bud induction frequency (95%) after three multiplication cycles was achieved on a GMD basal medium supplemented with 4.45 μM BAP and 0.05 μM NAA for three weeks. The axillary bud multiplication protocol for seedlings is potentially useful for large-scale propagation of seeds coming from the Portuguese breeding program of P. pinaster.
| Original language | English |
|---|---|
| Pages (from-to) | 24-33 |
| Number of pages | 10 |
| Journal | Propagation Of Ornamental Plants |
| Volume | 6 |
| Issue number | 1 |
| Publication status | Published - 12 Apr 2006 |
Keywords
- Axillary bud multiplication
- Maritime pine
- Micropropagation
- Organogenesis
- Plant regeneration