Improved axillary and adventitious bud regeneration from Portuguese genotypes of Pinus pinaster AIT

Susana Tereso, Sónia Gonçalves, Liliana Marum, Margarida Oliveira, João Maroco, Célia Miguel

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15 Citations (Scopus)


Axillary bud multiplication and direct adventitious bud regeneration were improved for clonal propagation of Portuguese genotypes of Pinus pinaster. Axillary buds induced in an ex vitro two year-old genotype by 6-benzylaminopurine (BAP) spraying and apex removal were used for the establishment of in vitro axillary bud cultures. Improved procedures were also developed for both axillary bud multiplication from four week-old seedlings and direct adventitious bud induction from cotyledons. The first in vitro multiplication cycle of buds derived from the ex vitro material resulted in an approximately five-fold multiplication rate. Regarding the axillary buds derived from four-week-old seedlings, the best induction medium for bud multiplication was based on a GD medium plus 1.11 μM BAP and 0.27 μM α- naphthaleneacetic acid (NAA). Induction of adventitious buds varied significantly among seed provenances. In the best performing provenance, the highest bud induction frequency (95%) after three multiplication cycles was achieved on a GMD basal medium supplemented with 4.45 μM BAP and 0.05 μM NAA for three weeks. The axillary bud multiplication protocol for seedlings is potentially useful for large-scale propagation of seeds coming from the Portuguese breeding program of P. pinaster.

Original languageEnglish
Pages (from-to)24-33
Number of pages10
JournalPropagation Of Ornamental Plants
Issue number1
Publication statusPublished - 12 Apr 2006


  • Axillary bud multiplication
  • Maritime pine
  • Micropropagation
  • Organogenesis
  • Plant regeneration


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