Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals

S. L. Vargas; C. Ponce; R. Bustamante; E. Calderón; G. Nevez; Y. De Armas; O. Matos; R. F. Miller; M. J. Gallo

doi:10.1007/s10096-017-3006-8

Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals

S. L. Vargas, C. Ponce, R. Bustamante, E. Calderón, G. Nevez, Y. De Armas, O. Matos, R. F. Miller, M. J. Gallo

Research output: Contribution to journal › Review article › peer-review

1 Citation (Scopus)

Abstract

To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.

Original language	English
Pages (from-to)	1711-1716
Number of pages	6
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	36
Issue number	10
DOIs	https://doi.org/10.1007/s10096-017-3006-8
Publication status	Published - 1 Oct 2017

Keywords

INFANT-DEATH-SYNDROME
F-SP HOMINIS
GENERAL-POPULATION
CARINII-PNEUMONIA
JIROVECII DNA
COLONIZATION
INFECTION

UN Sustainable Development Goals (Under maintenance)

SDG 3 - Good Health and Well-Being

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10.1007/s10096-017-3006-8

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Vargas, S. L., Ponce, C., Bustamante, R., Calderón, E., Nevez, G., De Armas, Y., Matos, O., Miller, R. F., & Gallo, M. J. (2017). Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. European Journal of Clinical Microbiology and Infectious Diseases, 36(10), 1711-1716. https://doi.org/10.1007/s10096-017-3006-8

Vargas, S. L. ; Ponce, C. ; Bustamante, R. ; Calderón, E. ; Nevez, G. ; De Armas, Y. ; Matos, O. ; Miller, R. F. ; Gallo, M. J. / Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. In: European Journal of Clinical Microbiology and Infectious Diseases. 2017 ; Vol. 36, No. 10. pp. 1711-1716.

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abstract = "To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.",

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Vargas, SL, Ponce, C, Bustamante, R, Calderón, E, Nevez, G, De Armas, Y, Matos, O, Miller, RF & Gallo, MJ 2017, 'Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals', European Journal of Clinical Microbiology and Infectious Diseases, vol. 36, no. 10, pp. 1711-1716. https://doi.org/10.1007/s10096-017-3006-8

Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. / Vargas, S. L.; Ponce, C.; Bustamante, R.; Calderón, E.; Nevez, G.; De Armas, Y.; Matos, O.; Miller, R. F.; Gallo, M. J.

In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 36, No. 10, 01.10.2017, p. 1711-1716.

Research output: Contribution to journal › Review article › peer-review

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T1 - Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals

AU - Vargas, S. L.

AU - Ponce, C.

AU - Bustamante, R.

AU - Calderón, E.

AU - Nevez, G.

AU - De Armas, Y.

AU - Matos, O.

AU - Miller, R. F.

AU - Gallo, M. J.

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AB - To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.

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KW - F-SP HOMINIS

KW - GENERAL-POPULATION

KW - CARINII-PNEUMONIA

KW - JIROVECII DNA

KW - COLONIZATION

KW - INFECTION

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AN - SCOPUS:85020196995

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EP - 1716

JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

SN - 0934-9723

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Vargas SL, Ponce C, Bustamante R, Calderón E, Nevez G, De Armas Y et al. Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. European Journal of Clinical Microbiology and Infectious Diseases. 2017 Oct 1;36(10):1711-1716. https://doi.org/10.1007/s10096-017-3006-8

Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals

Abstract

Keywords

UN Sustainable Development Goals (Under maintenance)

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