Impact of sludge retention time on MBR fouling

role of extracellular polymeric substances determined through membrane autopsy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The impact of sludge retention time (SRT) on the biofouling of a membrane bioreactor (MBR) by extracellular polymeric substances (EPS) was investigated. The MBR was operated at 60 and 20 d SRT. The gel layer (recovered through optimized membrane autopsy methods) and the cake layer were analyzed for their content and profile of EPS proteins and polysaccharides. The change to a shorter SRT led to decreased membrane filterability, concomitant with a higher expression of EPS proteins in the cake layer, which were identified as being mainly related with biosynthesis and stress functions. The gel layer was more substantial in internal fibers, with polysaccharides being the major component in this layer. With the decrease in SRT (and filterability decrease), the overall polysaccharide content and sugar variety increased. In conclusion, SRT impacted not only on the quantity but also the composition of EPS molecules, and both were shown to be important in biofouling.

Original languageEnglish
Pages (from-to)556-566
Number of pages11
JournalBiofouling
Volume33
Issue number7
DOIs
Publication statusPublished - 9 Aug 2017

Fingerprint

fouling
Bioreactors
bioreactors
Sewage
sludge
bioreactor
Autopsy
necropsy
membrane
Biofouling
filterability
Membranes
polysaccharide
Polysaccharides
biofouling
polysaccharides
gel
Gels
gels
protein

Keywords

  • cake layer
  • extracellular polymeric substances
  • gel layer
  • Membrane bioreactor
  • polysaccharides
  • proteins

Cite this

@article{785997af1d3e44928861a83fca8d27fa,
title = "Impact of sludge retention time on MBR fouling: role of extracellular polymeric substances determined through membrane autopsy",
abstract = "The impact of sludge retention time (SRT) on the biofouling of a membrane bioreactor (MBR) by extracellular polymeric substances (EPS) was investigated. The MBR was operated at 60 and 20 d SRT. The gel layer (recovered through optimized membrane autopsy methods) and the cake layer were analyzed for their content and profile of EPS proteins and polysaccharides. The change to a shorter SRT led to decreased membrane filterability, concomitant with a higher expression of EPS proteins in the cake layer, which were identified as being mainly related with biosynthesis and stress functions. The gel layer was more substantial in internal fibers, with polysaccharides being the major component in this layer. With the decrease in SRT (and filterability decrease), the overall polysaccharide content and sugar variety increased. In conclusion, SRT impacted not only on the quantity but also the composition of EPS molecules, and both were shown to be important in biofouling.",
keywords = "cake layer, extracellular polymeric substances, gel layer, Membrane bioreactor, polysaccharides, proteins",
author = "Silva, {Ana F.} and Silvia Antunes and Filomena Freitas and Gilda Carvalho and Reis, {Maria A.M.} and Crespo, {Maria T. Barreto}",
note = "This study was funded by Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) [project number PTDC/EBB-EBI/098862/2008], [grant number SFRH/BPD/97612/2013], [grant number SFRH/BD/80819/2011], [grant number SFRH/BPD/72280/2010]; iNOVA4Health Research Unit [grant number LISBOA-01-0145-FEDER-007344], cofunded by Fundacao para a Ciencia e Tecnologia/Ministerio da Ciencia e do Ensino Superior, through national funds, and by FEDER under the PT2020 Partnership Agreement, is acknowledged. Unity of Ciencias Biomoleculares Aplicadas-UCIBIO, financed by national funds from FCT/MEC [grant number UID/Multi/04378/2013] and co-financed by the ERDF under the PT2020 Partnership Agreement [grant number POCI-01-0145-FEDER-007728], is acknowledged.",
year = "2017",
month = "8",
day = "9",
doi = "10.1080/08927014.2017.1333112",
language = "English",
volume = "33",
pages = "556--566",
journal = "Biofouling",
issn = "0892-7014",
publisher = "Taylor & Francis",
number = "7",

}

TY - JOUR

T1 - Impact of sludge retention time on MBR fouling

T2 - role of extracellular polymeric substances determined through membrane autopsy

AU - Silva, Ana F.

AU - Antunes, Silvia

AU - Freitas, Filomena

AU - Carvalho, Gilda

AU - Reis, Maria A.M.

AU - Crespo, Maria T. Barreto

N1 - This study was funded by Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) [project number PTDC/EBB-EBI/098862/2008], [grant number SFRH/BPD/97612/2013], [grant number SFRH/BD/80819/2011], [grant number SFRH/BPD/72280/2010]; iNOVA4Health Research Unit [grant number LISBOA-01-0145-FEDER-007344], cofunded by Fundacao para a Ciencia e Tecnologia/Ministerio da Ciencia e do Ensino Superior, through national funds, and by FEDER under the PT2020 Partnership Agreement, is acknowledged. Unity of Ciencias Biomoleculares Aplicadas-UCIBIO, financed by national funds from FCT/MEC [grant number UID/Multi/04378/2013] and co-financed by the ERDF under the PT2020 Partnership Agreement [grant number POCI-01-0145-FEDER-007728], is acknowledged.

PY - 2017/8/9

Y1 - 2017/8/9

N2 - The impact of sludge retention time (SRT) on the biofouling of a membrane bioreactor (MBR) by extracellular polymeric substances (EPS) was investigated. The MBR was operated at 60 and 20 d SRT. The gel layer (recovered through optimized membrane autopsy methods) and the cake layer were analyzed for their content and profile of EPS proteins and polysaccharides. The change to a shorter SRT led to decreased membrane filterability, concomitant with a higher expression of EPS proteins in the cake layer, which were identified as being mainly related with biosynthesis and stress functions. The gel layer was more substantial in internal fibers, with polysaccharides being the major component in this layer. With the decrease in SRT (and filterability decrease), the overall polysaccharide content and sugar variety increased. In conclusion, SRT impacted not only on the quantity but also the composition of EPS molecules, and both were shown to be important in biofouling.

AB - The impact of sludge retention time (SRT) on the biofouling of a membrane bioreactor (MBR) by extracellular polymeric substances (EPS) was investigated. The MBR was operated at 60 and 20 d SRT. The gel layer (recovered through optimized membrane autopsy methods) and the cake layer were analyzed for their content and profile of EPS proteins and polysaccharides. The change to a shorter SRT led to decreased membrane filterability, concomitant with a higher expression of EPS proteins in the cake layer, which were identified as being mainly related with biosynthesis and stress functions. The gel layer was more substantial in internal fibers, with polysaccharides being the major component in this layer. With the decrease in SRT (and filterability decrease), the overall polysaccharide content and sugar variety increased. In conclusion, SRT impacted not only on the quantity but also the composition of EPS molecules, and both were shown to be important in biofouling.

KW - cake layer

KW - extracellular polymeric substances

KW - gel layer

KW - Membrane bioreactor

KW - polysaccharides

KW - proteins

UR - http://www.scopus.com/inward/record.url?scp=85021825805&partnerID=8YFLogxK

U2 - 10.1080/08927014.2017.1333112

DO - 10.1080/08927014.2017.1333112

M3 - Article

VL - 33

SP - 556

EP - 566

JO - Biofouling

JF - Biofouling

SN - 0892-7014

IS - 7

ER -