Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms

Diogo Queirós; Catarina Rangel; Paulo C. Lemos; Simona Rossetti; Luísa Seuanes Serafim

doi:10.3390/fermentation4030058

Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms

Diogo Queirós, Catarina Rangel, Paulo C. Lemos, Simona Rossetti, Luísa Seuanes Serafim

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Abstract

The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.

Original language	English
Article number	58
Journal	Fermentation
Volume	4
Issue number	3
DOIs	https://doi.org/10.3390/fermentation4030058
Publication status	Published - 24 Jul 2018

Keywords

Fluorescence in situ Hybridization (FISH)
Hardwood spent sulfite liquor
Mixed microbial cultures
Polyhydroxyalkanoates
Short-chain organic acids

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10.3390/fermentation4030058Licence: CC BY

fermentation-04-00058Final published version, 2.75 MBLicence: CC BY

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title = "Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms",

abstract = "The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.",

keywords = "Fluorescence in situ Hybridization (FISH), Hardwood spent sulfite liquor, Mixed microbial cultures, Polyhydroxyalkanoates, Short-chain organic acids",

author = "Diogo Queir{\'o}s and Catarina Rangel and Lemos, {Paulo C.} and Simona Rossetti and Serafim, {Lu{\'i}sa Seuanes}",

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AU - Lemos, Paulo C.

AU - Rossetti, Simona

AU - Serafim, Luísa Seuanes

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N2 - The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.

AB - The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.

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Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms

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Keywords

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