Impact of γ-rays on seed germination/short-term storage in four native alpine species

Correlation with free radical and antioxidant profiles

Deborah Zani, Daniele Dondi, Susana Araújo, Andrea Mondoni, Alma Balestrazzi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, the impact of gamma (γ) radiation on seeds was investigated in four native alpine species, Campanula barbata L., Cirsium spinosissinum (L.) Scop., Plantago alpina L., and Silene vulgaris (Moench) Garcke. Seeds were γ-irradiated with 100 and 200 Gy total doses delivered at a high dose rate of 2.7 Gy min−1. Irradiated and non-irradiated seeds were used immediately, and subsequently 7 and 14 days after drying (15% Relative Humidity, 15 °C) to assess their response to standard seed bank processing. Germination rates, seedling length and weight, antioxidant activity and phenolics content were measured, while free radical accumulation profiles were acquired by electron paramagnetic resonance (EPR). Germination was only hampered in irradiated C. barbata seeds. C. barbata and C. spinosissinum seedlings obtained from irradiated seeds suffered a decrease in length and weight, while growth was not affected in P. alpina and S. vulgaris, when compared to non-irradiated control. Although profiles of seed antioxidant activity were not influenced immediately after γ-irradiation, subsequent drying under seed bank standards induced changes in seed antioxidant activity, depending on the species. According to EPR data, C. barbata and C. Spinosissinum seeds revealed high free radical levels in non-irradiated samples, which were further enhanced by γ-irradiation. An opposite behaviour was observed in P. alpina and S. vulgaris. The four alpine species showed different profiles of γ-ray sensitivity. The reported data encourage future research to test inter-specific variability in the plant response to γ-rays based on a multidisciplinary approach which integrates environmental data. Considering that seeds of alpine plants are short-lived in storage, γ-irradiation could emerge as a promissory priming tool for native endangered species.

Original languageEnglish
Pages (from-to)86-94
Number of pages9
JournalRadiation Physics and Chemistry
Volume131
DOIs
Publication statusPublished - 1 Feb 2017

Fingerprint

germination
antioxidants
free radicals
seeds
rays
profiles
drying
irradiation
endangered species
electron paramagnetic resonance
priming
dosage
humidity

Keywords

  • Alpine species
  • Antioxidant activity
  • Electron Paramagnetic Resonance
  • Gamma rays
  • Seed germination

Cite this

@article{a38d229899cc4885a69d66c94f39b51a,
title = "Impact of γ-rays on seed germination/short-term storage in four native alpine species: Correlation with free radical and antioxidant profiles",
abstract = "In this study, the impact of gamma (γ) radiation on seeds was investigated in four native alpine species, Campanula barbata L., Cirsium spinosissinum (L.) Scop., Plantago alpina L., and Silene vulgaris (Moench) Garcke. Seeds were γ-irradiated with 100 and 200 Gy total doses delivered at a high dose rate of 2.7 Gy min−1. Irradiated and non-irradiated seeds were used immediately, and subsequently 7 and 14 days after drying (15{\%} Relative Humidity, 15 °C) to assess their response to standard seed bank processing. Germination rates, seedling length and weight, antioxidant activity and phenolics content were measured, while free radical accumulation profiles were acquired by electron paramagnetic resonance (EPR). Germination was only hampered in irradiated C. barbata seeds. C. barbata and C. spinosissinum seedlings obtained from irradiated seeds suffered a decrease in length and weight, while growth was not affected in P. alpina and S. vulgaris, when compared to non-irradiated control. Although profiles of seed antioxidant activity were not influenced immediately after γ-irradiation, subsequent drying under seed bank standards induced changes in seed antioxidant activity, depending on the species. According to EPR data, C. barbata and C. Spinosissinum seeds revealed high free radical levels in non-irradiated samples, which were further enhanced by γ-irradiation. An opposite behaviour was observed in P. alpina and S. vulgaris. The four alpine species showed different profiles of γ-ray sensitivity. The reported data encourage future research to test inter-specific variability in the plant response to γ-rays based on a multidisciplinary approach which integrates environmental data. Considering that seeds of alpine plants are short-lived in storage, γ-irradiation could emerge as a promissory priming tool for native endangered species.",
keywords = "Alpine species, Antioxidant activity, Electron Paramagnetic Resonance, Gamma rays, Seed germination",
author = "Deborah Zani and Daniele Dondi and Susana Ara{\'u}jo and Andrea Mondoni and Alma Balestrazzi",
year = "2017",
month = "2",
day = "1",
doi = "10.1016/j.radphyschem.2016.11.001",
language = "English",
volume = "131",
pages = "86--94",
journal = "Radiation Physics and Chemistry",
issn = "0969-806X",
publisher = "PERGAMON-ELSEVIER SCIENCE LTD",

}

Impact of γ-rays on seed germination/short-term storage in four native alpine species : Correlation with free radical and antioxidant profiles. / Zani, Deborah; Dondi, Daniele; Araújo, Susana; Mondoni, Andrea; Balestrazzi, Alma.

In: Radiation Physics and Chemistry, Vol. 131, 01.02.2017, p. 86-94.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impact of γ-rays on seed germination/short-term storage in four native alpine species

T2 - Correlation with free radical and antioxidant profiles

AU - Zani, Deborah

AU - Dondi, Daniele

AU - Araújo, Susana

AU - Mondoni, Andrea

AU - Balestrazzi, Alma

PY - 2017/2/1

Y1 - 2017/2/1

N2 - In this study, the impact of gamma (γ) radiation on seeds was investigated in four native alpine species, Campanula barbata L., Cirsium spinosissinum (L.) Scop., Plantago alpina L., and Silene vulgaris (Moench) Garcke. Seeds were γ-irradiated with 100 and 200 Gy total doses delivered at a high dose rate of 2.7 Gy min−1. Irradiated and non-irradiated seeds were used immediately, and subsequently 7 and 14 days after drying (15% Relative Humidity, 15 °C) to assess their response to standard seed bank processing. Germination rates, seedling length and weight, antioxidant activity and phenolics content were measured, while free radical accumulation profiles were acquired by electron paramagnetic resonance (EPR). Germination was only hampered in irradiated C. barbata seeds. C. barbata and C. spinosissinum seedlings obtained from irradiated seeds suffered a decrease in length and weight, while growth was not affected in P. alpina and S. vulgaris, when compared to non-irradiated control. Although profiles of seed antioxidant activity were not influenced immediately after γ-irradiation, subsequent drying under seed bank standards induced changes in seed antioxidant activity, depending on the species. According to EPR data, C. barbata and C. Spinosissinum seeds revealed high free radical levels in non-irradiated samples, which were further enhanced by γ-irradiation. An opposite behaviour was observed in P. alpina and S. vulgaris. The four alpine species showed different profiles of γ-ray sensitivity. The reported data encourage future research to test inter-specific variability in the plant response to γ-rays based on a multidisciplinary approach which integrates environmental data. Considering that seeds of alpine plants are short-lived in storage, γ-irradiation could emerge as a promissory priming tool for native endangered species.

AB - In this study, the impact of gamma (γ) radiation on seeds was investigated in four native alpine species, Campanula barbata L., Cirsium spinosissinum (L.) Scop., Plantago alpina L., and Silene vulgaris (Moench) Garcke. Seeds were γ-irradiated with 100 and 200 Gy total doses delivered at a high dose rate of 2.7 Gy min−1. Irradiated and non-irradiated seeds were used immediately, and subsequently 7 and 14 days after drying (15% Relative Humidity, 15 °C) to assess their response to standard seed bank processing. Germination rates, seedling length and weight, antioxidant activity and phenolics content were measured, while free radical accumulation profiles were acquired by electron paramagnetic resonance (EPR). Germination was only hampered in irradiated C. barbata seeds. C. barbata and C. spinosissinum seedlings obtained from irradiated seeds suffered a decrease in length and weight, while growth was not affected in P. alpina and S. vulgaris, when compared to non-irradiated control. Although profiles of seed antioxidant activity were not influenced immediately after γ-irradiation, subsequent drying under seed bank standards induced changes in seed antioxidant activity, depending on the species. According to EPR data, C. barbata and C. Spinosissinum seeds revealed high free radical levels in non-irradiated samples, which were further enhanced by γ-irradiation. An opposite behaviour was observed in P. alpina and S. vulgaris. The four alpine species showed different profiles of γ-ray sensitivity. The reported data encourage future research to test inter-specific variability in the plant response to γ-rays based on a multidisciplinary approach which integrates environmental data. Considering that seeds of alpine plants are short-lived in storage, γ-irradiation could emerge as a promissory priming tool for native endangered species.

KW - Alpine species

KW - Antioxidant activity

KW - Electron Paramagnetic Resonance

KW - Gamma rays

KW - Seed germination

UR - http://www.scopus.com/inward/record.url?scp=84995562583&partnerID=8YFLogxK

U2 - 10.1016/j.radphyschem.2016.11.001

DO - 10.1016/j.radphyschem.2016.11.001

M3 - Article

VL - 131

SP - 86

EP - 94

JO - Radiation Physics and Chemistry

JF - Radiation Physics and Chemistry

SN - 0969-806X

ER -