Immunization with Leishmania infantum released proteins confers partial protection against parasite infection with a predominant Th1 specific immune response

Ricardo Rosa, Cláudia S Marques, Olivia Roos Rodrigues, Gabriela M. Santos-Gomes

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, protective effect and immune response elicited by protein fractions LiRic1 (>75 kDa) and LiRic2 (<37 kDa) released by Leishmania infantum promastigotes were analysed in challenged BALB/c mice. Viable parasites were quantified in spleen and isolated CD4+ and CD8+ T cells were stimulated for evaluation of proliferative response and cytokine production. Immunization triggered 50.4-66.9% of parasite reduction. Stimulated CD4+ T cells from challenged animals revealed high proliferation. IL-12 and IFN-γ were released by CD4+ T cells whereas IL-4 and IL-10 were impaired. LiRic1 and LiRic2 immunization gave partial protection and a CD4+ Th1 response. LiRic2 generated IL-12 by CD8+ T cells pointing to its participation in protective response. These results encourage further research on the development of a vaccine that provides long-lasting protection against zoonotic visceral leishmaniasis.

Original languageEnglish
Pages (from-to)4525-4532
Number of pages8
JournalVaccine
VolumeVol. 25
Issue numbern.º 23
DOIs
Publication statusPublished - 6 Jun 2007

Keywords

  • CD4 T cells
  • CD8 T cells
  • Immunization
  • Leishmania infantum released proteins
  • Protective immune response

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