Identification of unamplified genomic DNA sequences using gold nanoparticle probes and a novel thin film photodetector

Rodrigo Ferrão de Paiva Martins; Pedro Baptista; Leonardo Bione Silva; Leandro Raniero; Gonçalo Dória; Ricardo Franco; E. Fortunato

doi:10.1016/j.jnoncryso1.2007.09.074

Identification of unamplified genomic DNA sequences using gold nanoparticle probes and a novel thin film photodetector

Rodrigo Ferrão de Paiva Martins, Pedro Baptista, Leonardo Bione Silva, Leandro Raniero, Gonçalo Dória, Ricardo Franco, E. Fortunato

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

This paper describes a novel colorimetric method for detection of nucleic acid targets in a homogeneous format with improved sensitivity by means of a system based on the combination of a tunable monochromatic light source and an amorphous/nanocrystalline silicon photodetector that detects color and light intensity changes undergone by samples/assays containing tailored gold nanoparticles probes. This new low cost, portable, fast and simple optoelectronic platform, with the possibility to be re-used, permits detection of at least 400 fentomole of specific DNA sequences without target or signal amplification and was applied to the rapid detection of human pathogens in large variety of clinical samples such as Mycobacterium tuberculosis.

Original language	English
Pages (from-to)	2580-2584
Number of pages	5
Journal	Journal of Non-Crystalline Solids
Volume	354
Issue number	19-25
DOIs	https://doi.org/10.1016/j.jnoncryso1.2007.09.074
Publication status	Published - May 2008

Keywords

Silicon
Biosensors
Sensors
Nanoparticles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jnoncryso1.2007.09.074

Cite this

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title = "Identification of unamplified genomic DNA sequences using gold nanoparticle probes and a novel thin film photodetector",

abstract = "This paper describes a novel colorimetric method for detection of nucleic acid targets in a homogeneous format with improved sensitivity by means of a system based on the combination of a tunable monochromatic light source and an amorphous/nanocrystalline silicon photodetector that detects color and light intensity changes undergone by samples/assays containing tailored gold nanoparticles probes. This new low cost, portable, fast and simple optoelectronic platform, with the possibility to be re-used, permits detection of at least 400 fentomole of specific DNA sequences without target or signal amplification and was applied to the rapid detection of human pathogens in large variety of clinical samples such as Mycobacterium tuberculosis.",

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language = "English",

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T1 - Identification of unamplified genomic DNA sequences using gold nanoparticle probes and a novel thin film photodetector

AU - Martins, Rodrigo Ferrão de Paiva

AU - Baptista, Pedro

AU - Silva, Leonardo Bione

AU - Raniero, Leandro

AU - Dória, Gonçalo

AU - Franco, Ricardo

AU - Fortunato, E.

PY - 2008/5

Y1 - 2008/5

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AB - This paper describes a novel colorimetric method for detection of nucleic acid targets in a homogeneous format with improved sensitivity by means of a system based on the combination of a tunable monochromatic light source and an amorphous/nanocrystalline silicon photodetector that detects color and light intensity changes undergone by samples/assays containing tailored gold nanoparticles probes. This new low cost, portable, fast and simple optoelectronic platform, with the possibility to be re-used, permits detection of at least 400 fentomole of specific DNA sequences without target or signal amplification and was applied to the rapid detection of human pathogens in large variety of clinical samples such as Mycobacterium tuberculosis.

KW - biosensors

KW - silicon

KW - sensors

KW - nanoparticles

KW - Silicon

KW - Biosensors

KW - Sensors

KW - Nanoparticles

U2 - 10.1016/j.jnoncryso1.2007.09.074

DO - 10.1016/j.jnoncryso1.2007.09.074

M3 - Article

SN - 0022-3093

VL - 354

SP - 2580

EP - 2584

JO - Journal of Non-Crystalline Solids

JF - Journal of Non-Crystalline Solids

IS - 19-25

ER -

Identification of unamplified genomic DNA sequences using gold nanoparticle probes and a novel thin film photodetector

Abstract

Keywords

UN SDGs

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