TY - JOUR
T1 - Identification of nontuberculous mycobacteria in clinical samples using molecular methods: a 3-year study.
AU - Couto, Isabel Maria dos Santos Leitão
AU - Machado, D
AU - Bettencourt, Miguel Viveiros
AU - Rodrigues , L
AU - Amaral, Leonard
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Nontuberculous mycobacteria (NTM) are being increasingly isolated in clinical laboratories and present technical and therapeutic challenges. In the present study, we report our experience with the identification of NTM received from 12 Lisbon hospitals over a 3-year period using GenoType Mycobacterium (CM/AS) assays (HAIN Lifescience GmbH, Nehren, Germany). Together, the two kits identified 96.6% of all NTM isolates tested. Among the 18 NTM species identified, Mycobacterium avium complex was the most frequent, although it accounted for only 34% of all NTM. Introducing these methods for the rapid identification of NTM highlights the importance of NTM as potential pathogens and assisted the selection of adequate therapy.
AB - Nontuberculous mycobacteria (NTM) are being increasingly isolated in clinical laboratories and present technical and therapeutic challenges. In the present study, we report our experience with the identification of NTM received from 12 Lisbon hospitals over a 3-year period using GenoType Mycobacterium (CM/AS) assays (HAIN Lifescience GmbH, Nehren, Germany). Together, the two kits identified 96.6% of all NTM isolates tested. Among the 18 NTM species identified, Mycobacterium avium complex was the most frequent, although it accounted for only 34% of all NTM. Introducing these methods for the rapid identification of NTM highlights the importance of NTM as potential pathogens and assisted the selection of adequate therapy.
U2 - 10.1111/j.1469-0691.2009.03076.x
DO - 10.1111/j.1469-0691.2009.03076.x
M3 - Article
C2 - 19832711
SN - 1198-743X
VL - 16
SP - 1161
EP - 1164
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
IS - 8
ER -
