Identification of active compounds in wheat straw extracts with allelopathic activity by HRGC‐MS and HRGC‐FTIR

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Abstract

The allelopathic effects of decomposing wheat straw have been known for some time. In spite of the ecological and economical importance of the subject, only a limited number of compounds have been asociated with the allelopathic activity of wheat straw. In this study the high resolution, speed of analysis, and sensitivity of capillary gas chromatography coupled with mass spectromtry (HRGC‐MS) and Fourier transform infrared spectrometry (HRGC‐MS‐FTIR) have been used in a systematic study of the composition of biologically active wheat straw extracts. Droplet counter‐current chromatography (DCCC) was used for fractionation and isolation of components. The extract components were analyzed as methyl derivatives and free hydroxyl groups were located by means of ethylation with diazoethane. Twenty compounds were identified, most of them being phenolic acids. Naphthoic acid, azelaic acid and 1,2,3,5‐tetrabromobenzene were also identified.

Original languageEnglish
Pages (from-to)550-554
Number of pages5
JournalJournal of High Resolution Chromatography
Volume13
Issue number8
DOIs
Publication statusPublished - 1 Jan 1990

Keywords

  • Allelopathy
  • Cereal straws
  • HRGC‐FTIR
  • HRGC‐MS
  • Phenollc acids
  • Wheat

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