Hydrogen sulfide oxidation

Adaptive changes in mitochondria of SW480 colorectal cancer cells upon exposure to hypoxia

Francesca Malagrinò, Karim Zuhra, Ludovica Mascolo, Daniela Mastronicola, João B. Vicente, Elena Forte, Alessandro Giuffrè

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Abstract

Hydrogen sulfide (H2S), a known inhibitor of cytochrome c oxidase (CcOX), plays a key signaling role in human (patho)physiology. H2S is synthesized endogenously and mainly metabolized by a mitochondrial sulfide-oxidizing pathway including sulfide:quinone oxidoreductase (SQR), whereby H2S-derived electrons are injected into the respiratory chain stimulating O2 consumption and ATP synthesis. Under hypoxic conditions, H2S has higher stability and is synthesized at higher levels with protective effects for the cell. Herein, working on SW480 colon cancer cells, we evaluated the effect of hypoxia on the ability of cells to metabolize H2S. The sulfide-oxidizing activity was assessed by high-resolution respirometry, measuring the stimulatory effect of sulfide on rotenone-inhibited cell respiration in the absence or presence of antimycin A. Compared to cells grown under normoxic conditions (air O2), cells exposed for 24 h to hypoxia (1% O2) displayed a 1.3-fold reduction in maximal sulfide-oxidizing activity and 2.7-fold lower basal O2 respiration. Based on citrate synthase activity assays, mitochondria of hypoxia-treated cells were 1.8-fold less abundant and displayed 1.4-fold higher maximal sulfide-oxidizing activity and 2.6-fold enrichment in SQR as evaluated by immunoblotting. We speculate that under hypoxic conditions mitochondria undergo these adaptive changes to protect cell respiration from H2S poisoning.

Original languageEnglish
Article number8102936
JournalOxidative Medicine and Cellular Longevity
Volume2019
DOIs
Publication statusPublished - 1 Jan 2019

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Hydrogen Sulfide
Mitochondria
Sulfides
sulfide quinone reductase
Colorectal Neoplasms
Cells
Oxidation
Cell Respiration
Antimycin A
Rotenone
Cell Hypoxia
Citrate (si)-Synthase
Physiology
Electron Transport Complex IV
Electron Transport
Immunoblotting
Colonic Neoplasms
Poisoning
Assays
Respiration

Cite this

Malagrinò, Francesca ; Zuhra, Karim ; Mascolo, Ludovica ; Mastronicola, Daniela ; Vicente, João B. ; Forte, Elena ; Giuffrè, Alessandro. / Hydrogen sulfide oxidation : Adaptive changes in mitochondria of SW480 colorectal cancer cells upon exposure to hypoxia. In: Oxidative Medicine and Cellular Longevity. 2019 ; Vol. 2019.
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Hydrogen sulfide oxidation : Adaptive changes in mitochondria of SW480 colorectal cancer cells upon exposure to hypoxia. / Malagrinò, Francesca; Zuhra, Karim; Mascolo, Ludovica; Mastronicola, Daniela; Vicente, João B.; Forte, Elena; Giuffrè, Alessandro.

In: Oxidative Medicine and Cellular Longevity, Vol. 2019, 8102936, 01.01.2019.

Research output: Contribution to journalArticle

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T2 - Adaptive changes in mitochondria of SW480 colorectal cancer cells upon exposure to hypoxia

AU - Malagrinò, Francesca

AU - Zuhra, Karim

AU - Mascolo, Ludovica

AU - Mastronicola, Daniela

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AU - Giuffrè, Alessandro

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