Hybrid modeling of microbial exopolysaccharide (EPS) production: The case of Enterobacter A47

Rodolfo Marques, Moritz von Stosch, Rui M. C. Portela, Cristiana A. V. Torres, Sílvia Antunes, Filomena Freitas, Maria A.M. Reis, Rui Oliveira

Research output: Contribution to journalArticle

2 Citations (Scopus)


Enterobacter A47 is a bacterium that produces high amounts of a fucose-rich exopolysaccharide (EPS) from glycerol residue of the biodiesel industry. The fed-batch process is characterized by complex non-linear dynamics with highly viscous pseudo-plastic rheology due to the accumulation of EPS in the culture medium. In this paper, we study hybrid modeling as a methodology to increase the predictive power of models for EPS production optimization. We compare six hybrid structures that explore different levels of knowledge-based and machine-learning model components. Knowledge-based components consist of macroscopic material balances, Monod type kinetics, cardinal temperature and pH (CTP) dependency and power-law viscosity models. Unknown dependencies are set to be identified by a feedforward artificial neural network (ANN). A semiparametric identification schema is applied resorting to a data set of 13 independent fed-batch experiments. A parsimonious hybrid model was identified that describes the dynamics of the 13 experiments with the same parameterization. The final model is specific to Enterobacter A47 but can be easily extended to other microbial EPS processes.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalJournal of Biotechnology
Publication statusPublished - 20 Mar 2017


  • Enterobacter A47
  • Hybrid modeling
  • Knowledge-based modeling
  • Machine learning
  • Microbial exopolysaccharides (EPS)
  • Semiparametric identification

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