Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence

F.M. Pereira, Iwona Bernacka-Wojcik, R.S.R. Ribeiro, Maria Teresa Lobato, E. Fortunato, Rodrigo Ferrão de Paiva Martins, R. Igreja, Pedro A S Jorge, H. Águas, A.M.G. Oliva

Research output: Contribution to journalArticle

1 Citation (Scopus)
13 Downloads (Pure)

Abstract

This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting
Original languageEnglish
Article number181
JournalMicromachines
Volume7
Issue number10
DOIs
Publication statusPublished - 7 Oct 2016

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Acoustic impedance
Microfluidics
Light absorption
Fluorescence
Microelectrodes
Microfabrication
Photoresists
Labels
Optical fibers
Refractive index
Membranes
baysilon

Keywords

  • single cell analysis
  • label-free methods
  • impedance spectroscopy
  • refractometry
  • optical absorption
  • hybrid microfluidic chip

Cite this

@article{e2104570614448cc98092a3eadb7dc89,
title = "Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence",
abstract = "This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting",
keywords = "single cell analysis, label-free methods, impedance spectroscopy, refractometry, optical absorption, hybrid microfluidic chip",
author = "F.M. Pereira and Iwona Bernacka-Wojcik and R.S.R. Ribeiro and Lobato, {Maria Teresa} and E. Fortunato and Martins, {Rodrigo Ferr{\~a}o de Paiva} and R. Igreja and Jorge, {Pedro A S} and H. {\'A}guas and A.M.G. Oliva",
note = "FEDER funds through the COMPETE 2020 Programme National Funds through Fundacao para a Ciencia e a Tecnologia (FCT) (UID/CTM/50025/2013, PTDC/SAU-BEB/102247/2008) project {"}Project{"} NanoSTIMA: Macro-to-Nano Human Sensing: Towards Integrated Multimodal Health Monitoring and Analytics - North Portugal Regional Operational Programme (NORTE-01-0145-FEDER-000016) European Regional Development Fund (ERDF) North Portugal Regional Operational Program under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) (NORTE-07-0124-FEDER-000058) national funds, through the Portuguese funding agency, FCT",
year = "2016",
month = "10",
day = "7",
doi = "10.3390/mi7100181",
language = "English",
volume = "7",
journal = "Micromachines",
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}

Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence. / Pereira, F.M.; Bernacka-Wojcik, Iwona; Ribeiro, R.S.R.; Lobato, Maria Teresa; Fortunato, E.; Martins, Rodrigo Ferrão de Paiva; Igreja, R.; Jorge, Pedro A S; Águas, H.; Oliva, A.M.G.

In: Micromachines, Vol. 7, No. 10, 181, 07.10.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence

AU - Pereira, F.M.

AU - Bernacka-Wojcik, Iwona

AU - Ribeiro, R.S.R.

AU - Lobato, Maria Teresa

AU - Fortunato, E.

AU - Martins, Rodrigo Ferrão de Paiva

AU - Igreja, R.

AU - Jorge, Pedro A S

AU - Águas, H.

AU - Oliva, A.M.G.

N1 - FEDER funds through the COMPETE 2020 Programme National Funds through Fundacao para a Ciencia e a Tecnologia (FCT) (UID/CTM/50025/2013, PTDC/SAU-BEB/102247/2008) project "Project" NanoSTIMA: Macro-to-Nano Human Sensing: Towards Integrated Multimodal Health Monitoring and Analytics - North Portugal Regional Operational Programme (NORTE-01-0145-FEDER-000016) European Regional Development Fund (ERDF) North Portugal Regional Operational Program under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) (NORTE-07-0124-FEDER-000058) national funds, through the Portuguese funding agency, FCT

PY - 2016/10/7

Y1 - 2016/10/7

N2 - This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting

AB - This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting

KW - single cell analysis

KW - label-free methods

KW - impedance spectroscopy

KW - refractometry

KW - optical absorption

KW - hybrid microfluidic chip

U2 - 10.3390/mi7100181

DO - 10.3390/mi7100181

M3 - Article

VL - 7

JO - Micromachines

JF - Micromachines

IS - 10

M1 - 181

ER -