How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial

Hugo M. Santos, Luís B. Carvalho, Carlos Lodeiro, Gonçalo Martins, Inês L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, António P. A. Matos, J. L. Capelo

Research output: Contribution to journalReview articlepeer-review

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Abstract

The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.

Original languageEnglish
Article number108323
Number of pages10
JournalMicrochemical Journal
Volume186
Early online dateDec 2022
DOIs
Publication statusPublished - Mar 2023

Keywords

  • Immuno-mass spectrometry
  • LC-ESI-Q-TOF-MS/MS
  • MALDI-TOF-MS
  • Viral detection

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