TY - JOUR
T1 - How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry
T2 - A tutorial
AU - Santos, Hugo M.
AU - Carvalho, Luís B.
AU - Lodeiro, Carlos
AU - Martins, Gonçalo
AU - Gomes, Inês L.
AU - Antunes, Wilson D. T.
AU - Correia, Vanessa
AU - Almeida-Santos, Maria M.
AU - Rebelo-de-Andrade, Helena
AU - Matos, António P. A.
AU - Capelo, J. L.
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0008%2F2020/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F144222%2F2019/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F139384%2F2018/PT#
Funding Information:
PROTEOMASS Scientific Society is acknowledged by the funding provided to the Laboratory for Biological Mass Spectrometry Isabel Moura (#PM001/2019 and #PM003/2016). The authors acknowledge Dr. Miguel Fevereiro from INIAV for his valuable help and fruitful discussions.
Publisher Copyright:
© 2022 The Author(s)
PY - 2023/3
Y1 - 2023/3
N2 - The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.
AB - The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.
KW - Immuno-mass spectrometry
KW - LC-ESI-Q-TOF-MS/MS
KW - MALDI-TOF-MS
KW - Viral detection
UR - http://www.scopus.com/inward/record.url?scp=85144786406&partnerID=8YFLogxK
U2 - 10.1016/j.microc.2022.108323
DO - 10.1016/j.microc.2022.108323
M3 - Review article
AN - SCOPUS:85144786406
SN - 0026-265X
VL - 186
JO - Microchemical Journal
JF - Microchemical Journal
M1 - 108323
ER -