HIV-1 gp120 N-linked glycosylation differs between plasma and leukocyte compartments

Yung Shwen Ho, Ana B Abecasis, Kristof Theys, Koen Deforche, Dominic E Dwyer, Michael Charleston, Anne Mieke Vandamme, Nitin K Saksena

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


BACKGROUND: N-linked glycosylation is a major mechanism for minimizing virus neutralizing antibody response and is present on the Human Immunodeficiency Virus (HIV) envelope glycoprotein. Although it is known that glycosylation changes can dramatically influence virus recognition by the host antibody, the actual contribution of compartmental differences in N-linked glycosylation patterns remains unclear.

METHODOLOGY AND PRINCIPAL FINDINGS: We amplified the env gp120 C2-V5 region and analyzed 305 clones derived from plasma and other compartments from 15 HIV-1 patients. Bioinformatics and Bayesian network analyses were used to examine N-linked glycosylation differences between compartments. We found evidence for cellspecific single amino acid changes particular to monocytes, and significant variation was found in the total number of N-linked glycosylation sites between patients. Further, significant differences in the number of glycosylation sites were observed between plasma and cellular compartments. Bayesian network analyses showed an interdependency between N-linked glycosylation sites found in our study, which may have immense functional relevance.

CONCLUSION: Our analyses have identified single cell/compartment-specific amino acid changes and differences in N-linked glycosylation patterns between plasma and diverse blood leukocytes. Bayesian network analyses showed associations inferring alternative glycosylation pathways. We believe that these studies will provide crucial insights into the host immune response and its ability in controlling HIV replication in vivo. These findings could also have relevance in shielding and evasion of HIV-1 from neutralizing antibodies.

Original languageEnglish
Article number14
Pages (from-to)14-24
Number of pages10
JournalVirology Journal
VolumeVol. 5
Publication statusPublished - 23 Jan 2008


  • Amino Acids
  • Anti-HIV Agents
  • Antiretroviral Therapy, Highly Active
  • Bayes Theorem
  • Genetic Variation
  • Glycosylation
  • HIV Envelope Protein gp120
  • HIV Infections
  • HIV-1
  • Humans
  • Leukocytes
  • Monocytes
  • Phylogeny
  • Plasma
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't


Dive into the research topics of 'HIV-1 gp120 N-linked glycosylation differs between plasma and leukocyte compartments'. Together they form a unique fingerprint.

Cite this