TY - JOUR
T1 - Highly complex substrates lead to dynamic bacterial community for polyhydroxyalkanoates production
AU - Queirós, Diogo
AU - Fonseca, Alexandre
AU - Rossetti, Simona
AU - Serafim, Luísa S.
AU - Lemos, P. C.
N1 - This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013) and LAQV-REQUIMTE (FCT UID/QUI/50006/2013), financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265). Authors acknowledge Eng. A. Prates from CAIMA, Industria de Celulose S.A., Constancia, Portugal for HSSL. Diogo Queiros thanks FCT for his PhD Grant (SFRH/BD/87758/2012).
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates’ (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE. The community was found to be extremely dynamic, dominated by Alphaproteobacteria, with Paracoccus and Rhodobacter present, both PHA-storing microorganisms. 16S rRNA gene clone library further revealed that side populations’ non-PHA accumulators were able to strive (Agrobacterium, Flavobacteria, and Brachymonas). Nevertheless, reactor performance in terms of PHA storage was stable during operation time. The monitoring of the MMC population evolution provided information on the relation between community structure and process operation.
AB - Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates’ (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE. The community was found to be extremely dynamic, dominated by Alphaproteobacteria, with Paracoccus and Rhodobacter present, both PHA-storing microorganisms. 16S rRNA gene clone library further revealed that side populations’ non-PHA accumulators were able to strive (Agrobacterium, Flavobacteria, and Brachymonas). Nevertheless, reactor performance in terms of PHA storage was stable during operation time. The monitoring of the MMC population evolution provided information on the relation between community structure and process operation.
KW - Bacterial community dynamics
KW - Hardwood spent sulfite liquor (HSSL)
KW - Mixed microbial cultures (MMC)
KW - Molecular methods
KW - Polyhydroxyalkanoates (PHA)
UR - http://www.scopus.com/inward/record.url?scp=85018683303&partnerID=8YFLogxK
U2 - 10.1007/s10295-017-1951-y
DO - 10.1007/s10295-017-1951-y
M3 - Article
C2 - 28497282
AN - SCOPUS:85018683303
SN - 1367-5435
VL - 44
SP - 1215
EP - 1224
JO - Journal of Industrial Microbiology and Biotechnology
JF - Journal of Industrial Microbiology and Biotechnology
IS - 8
ER -