High-throughput sequence typing of T-cell epitope polymorphisms in Plasmodium falciparum circumsporozoite protein

Ali Alloueche, Henrique Silveira, David J. Conway, Kalifa Bojang, Tom Doherty, Joe Cohen, Margaret Pinder, Brian M. Greenwood

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We report a method for typing polymorphisms at the T-cell epitopes within the Th2R and Th3R regions of the Plasmodium falciparum circumsporozoite protein (CSP). This method combines the use of PCR and sequence specific oligonucleotide probes (PCR-SSOP), and allows the identification of single nucleotide polymorphisms in these epitope regions. PCR-SSOP is a robust and a high-throughput sequence typing technique which has the same specificity and fidelity as direct sequencing. This method has been developed specifically for the assessment of the protective efficacy of RTS,S/SBAS2 vaccine against the 3D7 strain of P. falciparum (RTS,S/SBAS2 vaccine contains a part of the 3D7 CSP protein) in a phase IIb trial in Gambia which has been completed recently. PCR-SSOP could be used to determine the allelic frequencies of other parasite antigens and their geographical distribution. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)273-282
Number of pages10
JournalMolecular And Biochemical Parasitology
VolumeVol. 106
Issue numbern.º 2
DOIs
Publication statusPublished - 5 Mar 2000

Keywords

  • Circumsporozoite protein
  • Malaria
  • PCR-SSOP
  • RTS,S/SBAS2 vaccine
  • Th2R/Th3R polymorphisms

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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