High storage of PHB by mixed microbial cultures under aerobic dynamic feeding conditions

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Activated sludge submitted to aerobic dynamic feeding conditions showed a high and stable capacity to store polyhydroxybutyrate (PHB). The influence of carbon and nitrogen concentrations on the PHB accumulation yield was studied in a range of 30 Cmmol/l to 180 Cmmol/l for acetate and between 0 Nmmol/l and 2.8 Nmmol/l for ammonia. Low ammonia concentrations favored PHB accumulation. The maximum PHB content, 67.5%, was obtained for 180 Cmmol/l of acetate supplied in one pulse but with a slower PHB storage rate. In order to avoid substrate inhibition, 180 Cmmol/l of acetate were supplied in two different ways: continuously fed and in three pulses of 60 Cmmol/l each. In both cases the specific PHB storage rate increased and the PHB content obtained were, 56.2% and 78.5%,respectively. Addition of acetate by pulses controlled by the oxygen concentration was kept for 16 days. The PHB content was always above 70% of cell dry weight.
Original languageEnglish
Title of host publicationEuropean Symposium on Environmental Biotechnology, ESEB 2004
EditorsW. Verstraete
PublisherA.A.Balkema Publishers
ISBN (Print)90-5809-653-X
Publication statusPublished - 2004
EventEuropean Symposium on Environmental Biotechnology - Oostende, Belgium
Duration: 25 Apr 200428 Apr 2004


ConferenceEuropean Symposium on Environmental Biotechnology

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