Abstract
Background: Staphylococcus pseudintermedius is the agent of skin and soft-tissue infections (SSTIs) in companion animals, for which antimicrobial resistance is a public health concern.
Objective: To characterize a collection of S. pseudintermedius causing SSTIs in companion animals, establishing the main clonal lineages and antimicrobial resistance traits.
Methods: The collection corresponded to all S. pseudintermedius (n=155) causing SSTIs in companion animals (dogs, cats and one rabbit) collected over five years at two laboratories in Lisbon, Portugal. Susceptibility patterns were established by disk diffusion for 28 antimicrobials from 15 classes. All isolates were typed by PFGE-SmaI typing and representative isolates further typed by MLST.
Results: Forty-eight isolates (31.0%) were methicillin-resistant (mecA+, MRSP). Multidrug-resistant (MDR) phenotypes were detected for 95.8% of the MRSP isolates and 22.4% methicillin-susceptible S. pseudintermedius (MSSP) isolates. Only 12.3% isolates were susceptible to all antimicrobials tested. In total, we detected 43 different antimicrobial resistance profiles. The 155 isolates were distributed within 129 PFGE clusters, grouped by MLST in 42 clonal lineages, 25 of which corresponding to new STs. ST71 was the most frequently lineage found, but other relevant lineages, including ST258, are described for the first time in Portugal.
Conclusions: This study evidence high rates of MDR profiles and MRSP associated with SSTIs in companion animals. The high clonal diversity of this collection linked to a significant antimicrobial resistance burden reinforces the need for a continuous surveillance of this pathogen.
Funding: Project BIOSAFE funded by FEDER/FCT (Portugal), Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017. Further support by FCT to GHTM (UID/04413/2020), CM and CF (Grants UI/BD/151061/2021 and 2021.05063.BD).
Objective: To characterize a collection of S. pseudintermedius causing SSTIs in companion animals, establishing the main clonal lineages and antimicrobial resistance traits.
Methods: The collection corresponded to all S. pseudintermedius (n=155) causing SSTIs in companion animals (dogs, cats and one rabbit) collected over five years at two laboratories in Lisbon, Portugal. Susceptibility patterns were established by disk diffusion for 28 antimicrobials from 15 classes. All isolates were typed by PFGE-SmaI typing and representative isolates further typed by MLST.
Results: Forty-eight isolates (31.0%) were methicillin-resistant (mecA+, MRSP). Multidrug-resistant (MDR) phenotypes were detected for 95.8% of the MRSP isolates and 22.4% methicillin-susceptible S. pseudintermedius (MSSP) isolates. Only 12.3% isolates were susceptible to all antimicrobials tested. In total, we detected 43 different antimicrobial resistance profiles. The 155 isolates were distributed within 129 PFGE clusters, grouped by MLST in 42 clonal lineages, 25 of which corresponding to new STs. ST71 was the most frequently lineage found, but other relevant lineages, including ST258, are described for the first time in Portugal.
Conclusions: This study evidence high rates of MDR profiles and MRSP associated with SSTIs in companion animals. The high clonal diversity of this collection linked to a significant antimicrobial resistance burden reinforces the need for a continuous surveillance of this pathogen.
Funding: Project BIOSAFE funded by FEDER/FCT (Portugal), Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017. Further support by FCT to GHTM (UID/04413/2020), CM and CF (Grants UI/BD/151061/2021 and 2021.05063.BD).
| Original language | English |
|---|---|
| Publication status | Published - 21 Apr 2023 |
| Event | 6º Congresso Nacional de Medicina Tropical - IHMT, Lisboa, Portugal Duration: 20 Apr 2023 → 21 Apr 2023 https://6cnmt.admeus.pt/?page=43 |
Conference
| Conference | 6º Congresso Nacional de Medicina Tropical |
|---|---|
| Abbreviated title | 6º CNMT |
| Country/Territory | Portugal |
| City | Lisboa |
| Period | 20/04/23 → 21/04/23 |
| Internet address |
