A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation. Bioaffinity conjugation of the biotin/avidin system was subsequently exploited for further self-assembly avidin family proteins onto photobiotin arrays. The excellent fouling release PFPEs surface properties enable performing avidin assembly step simply by arrays incubation without PFPEs surface passivation or chemical modification to avoid unspecific biomolecule adsorption. Finally, as a proof of principle biotinylated heparin was successfully grafted onto photobiotin/avidin arrays.
- Microcontact arrayer
- Microcontact printing
- Soft lithography
Credi, C., De Marco, C., Molena, E., Pla Roca, M., Samitier Martí, J., Marques, J., Fernàndez-Busquets, X., Levi, M., & Turri, S. (2016). Heparin micropatterning onto fouling-release perfluoropolyether-based polymers via photobiotin activation. Colloids and Surfaces B: Biointerfaces, Vol. 146, 250-259. https://doi.org/10.1016/j.colsurfb.2016.06.020