Heparin micropatterning onto fouling-release perfluoropolyether-based polymers via photobiotin activation

Caterina Credi, Carmela De Marco, Elena Molena, Mateu Pla Roca, Josep Samitier Martí, Joana Marques, Xavier Fernàndez-Busquets, Marinella Levi, Stefano Turri

Research output: Contribution to journalArticle

Abstract

A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation. Bioaffinity conjugation of the biotin/avidin system was subsequently exploited for further self-assembly avidin family proteins onto photobiotin arrays. The excellent fouling release PFPEs surface properties enable performing avidin assembly step simply by arrays incubation without PFPEs surface passivation or chemical modification to avoid unspecific biomolecule adsorption. Finally, as a proof of principle biotinylated heparin was successfully grafted onto photobiotin/avidin arrays.
Original languageEnglish
Pages (from-to)250-259
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
VolumeVol. 146
DOIs
Publication statusPublished - 1 Oct 2016

Keywords

  • Antifouling
  • Heparin
  • Malaria
  • Microcontact arrayer
  • Microcontact printing
  • Micropatterning
  • Perfluoropolyether
  • Photobiotin
  • Polymers
  • Soft lithography

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    Credi, C., De Marco, C., Molena, E., Pla Roca, M., Samitier Martí, J., Marques, J., Fernàndez-Busquets, X., Levi, M., & Turri, S. (2016). Heparin micropatterning onto fouling-release perfluoropolyether-based polymers via photobiotin activation. Colloids and Surfaces B: Biointerfaces, Vol. 146, 250-259. https://doi.org/10.1016/j.colsurfb.2016.06.020