TY - JOUR
T1 - HBM4EU Chromates Study
T2 - urinary metabolomics study of workers exposed to hexavalent chromium
AU - Kozłowska, Lucyna
AU - Santonen, Tiina
AU - Duca, Radu Corneliu
AU - Godderis, Lode
AU - Jagiello, Karolina
AU - Janasik, Beata
AU - Van Nieuwenhuyse, An
AU - Poels, Katrien
AU - Puzyn, Tomasz
AU - Scheepers, Paul T J
AU - Sijko, Monika
AU - Silva, Maria João
AU - Sosnowska, Anita
AU - Viegas, Susana
AU - Verdonck, Jelle
AU - Wąsowicz, Wojciech
AU - On Behalf Of Hbm Eu Chromates Study Team, null
AU - On Behalf Of Statistical Team, null
PY - 2022/4/18
Y1 - 2022/4/18
N2 - Exposure to hexavalent chromium Cr(VI) may occur in several occupational activities, placing workers in many industries at risk for potential related health outcomes. Untargeted metabolomics was applied to investigate changes in metabolic pathways in response to Cr(VI) exposure. We obtained our data from a study population of 220 male workers with exposure to Cr(VI) and 102 male controls from Belgium, Finland, Poland, Portugal and the Netherlands within the HBM4EU Chromates Study. Urinary metabolite profiles were determined using liquid chromatography mass spectrometry, and differences between post-shift exposed workers and controls were analyzed using principal component analysis. Based on the first two principal components, we observed clustering by industrial chromate application, such as welding, chrome plating, and surface treatment, distinct from controls and not explained by smoking status or alcohol use. The changes in the abundancy of excreted metabolites observed in workers reflect fatty acid and monoamine neurotransmitter metabolism, oxidative modifications of amino acid residues, the excessive formation of abnormal amino acid metabolites and changes in steroid and thyrotropin-releasing hormones. The observed responses could also have resulted from work-related factors other than Cr(VI). Further targeted metabolomics studies are needed to better understand the observed modifications and further explore the suitability of urinary metabolites as early indicators of adverse effects associated with exposure to Cr(VI).
AB - Exposure to hexavalent chromium Cr(VI) may occur in several occupational activities, placing workers in many industries at risk for potential related health outcomes. Untargeted metabolomics was applied to investigate changes in metabolic pathways in response to Cr(VI) exposure. We obtained our data from a study population of 220 male workers with exposure to Cr(VI) and 102 male controls from Belgium, Finland, Poland, Portugal and the Netherlands within the HBM4EU Chromates Study. Urinary metabolite profiles were determined using liquid chromatography mass spectrometry, and differences between post-shift exposed workers and controls were analyzed using principal component analysis. Based on the first two principal components, we observed clustering by industrial chromate application, such as welding, chrome plating, and surface treatment, distinct from controls and not explained by smoking status or alcohol use. The changes in the abundancy of excreted metabolites observed in workers reflect fatty acid and monoamine neurotransmitter metabolism, oxidative modifications of amino acid residues, the excessive formation of abnormal amino acid metabolites and changes in steroid and thyrotropin-releasing hormones. The observed responses could also have resulted from work-related factors other than Cr(VI). Further targeted metabolomics studies are needed to better understand the observed modifications and further explore the suitability of urinary metabolites as early indicators of adverse effects associated with exposure to Cr(VI).
U2 - 10.3390/metabo12040362
DO - 10.3390/metabo12040362
M3 - Article
C2 - 35448548
SN - 2218-1989
VL - 12
JO - Metabolites
JF - Metabolites
IS - 4
M1 - 362
ER -
