TY - JOUR
T1 - Haplotype diversity patterns in Quercus suber (Fagaceae) inferred from cpDNA sequence data
AU - Sousa, Filipe
AU - Viegas, Mariana Bray
AU - Costa, Joana
AU - Marques, Isabel
AU - Pina-Martins, Francisco
AU - Simões, Fernanda
AU - Matos, José
AU - Glushkova, Maria
AU - Miguel, Célia
AU - Veloso, Maria Manuela
AU - Oliveira, M. Margarida
AU - Ricardo, Cândido Pinto
AU - Batista, Dora
AU - Paulo, Octávio S.
N1 - Funding Information:
This work was supported by project ‘Keep Pace: Selection of trees keeping pace with fast environmental changes, a science-based approach for sustainable XXI century Oak forests’, co-funded by the EU ERDF funds, within the Portugal 2020 Partnership Agreement and Programa Operacional Regional Algarve, and by national funds through Fundação para a Ciência e Tecnologia (FCT) Portugal (ALG-01-0145-FEDER-029263 / PTDC/ASP/SIL/29263/2017). Additional support was received through grants PTDC/AGR-GPL/104966/2008 and SOBREIRO/0036/2009, and through the research units GREEN-IT, Bioresources for Sustainability (UIDB/04551/2020) and cE3c (UIDB/00329/2020).
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - Chloroplast genome diversity in cork oak (Quercus suber) is characterised by the occurrence of haplotypes that are akin to those found in other Mediterranean oak species, particularly in Q. ilex and Q. rotundifolia, suggesting the possible presence of an introgressed chloroplast lineage. To further investigate this pattern, we reconstructed chloroplast haplotypes by sequencing four chloroplast markers (cpDNA), sampled across 181 individuals and 10 taxa. Our analyses resulted in the identification of two diversified chloroplast haplogroups in Q. suber, corresponding to a geographically widespread lineage and an Afro-Iberian lineage. Time-calibrated phylogenetic analyses of cpDNA point to a Miocene origin of the two haplogroups in Q. suber, suggesting that the Afro-Iberian lineage was present in cork oak before the onset of glaciation periods. The persistence of the two haplogroups in the western part of the species distribution range may be a consequence of either ancient introgression events or chloroplast lineage sorting, combined with different fixation in refugia through glaciation periods. Our results provide a comprehensive insight on the origins of chloroplast diversity in these ecologically and economically important Mediterranean oaks.
AB - Chloroplast genome diversity in cork oak (Quercus suber) is characterised by the occurrence of haplotypes that are akin to those found in other Mediterranean oak species, particularly in Q. ilex and Q. rotundifolia, suggesting the possible presence of an introgressed chloroplast lineage. To further investigate this pattern, we reconstructed chloroplast haplotypes by sequencing four chloroplast markers (cpDNA), sampled across 181 individuals and 10 taxa. Our analyses resulted in the identification of two diversified chloroplast haplogroups in Q. suber, corresponding to a geographically widespread lineage and an Afro-Iberian lineage. Time-calibrated phylogenetic analyses of cpDNA point to a Miocene origin of the two haplogroups in Q. suber, suggesting that the Afro-Iberian lineage was present in cork oak before the onset of glaciation periods. The persistence of the two haplogroups in the western part of the species distribution range may be a consequence of either ancient introgression events or chloroplast lineage sorting, combined with different fixation in refugia through glaciation periods. Our results provide a comprehensive insight on the origins of chloroplast diversity in these ecologically and economically important Mediterranean oaks.
KW - Glacial refugia
KW - Introgression
KW - Lineage sorting
KW - Mediterranean
KW - Oak forests
KW - Phylogeography
UR - http://www.scopus.com/inward/record.url?scp=85176797376&partnerID=8YFLogxK
U2 - 10.1007/s00606-023-01879-7
DO - 10.1007/s00606-023-01879-7
M3 - Article
AN - SCOPUS:85176797376
SN - 0378-2697
VL - 309
JO - Plant Systematics And Evolution
JF - Plant Systematics And Evolution
IS - 6
M1 - 42
ER -