H-1-NMR analysis of water mobility in cultured phototrophic biofilms

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Abstract

The present work reports on the first attempt to study water mobility in phototrophic biofilms, applying the (HNMR)-H-1 relaxometry technique to closely monitored microbial communities grown in a microcosm under controlled ambient conditions. Longitudinal water proton relaxation times exhibited a bi-exponential behavior in all biofilm samples, indicating two types of water molecules with diverging dynamic properties, confined to different compartments of the biofilm. The fast-relaxing component can be attributed to water molecules tightly bound to the intracellular matrix, while the slow-relaxing component could reflect the behavior of water embedded in the biopolymer matrix, confined into matrix pores and channels. The results are discussed with respect to a possible key role of exopolysaccharides and uronic acids in water binding in phototrophic biofilms.
Original languageUnknown
Pages (from-to)327-336
JournalBiofouling
Volume27
Issue number3
DOIs
Publication statusPublished - 1 Jan 2011

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