Green Synthesis and Antibacterial Activity of Silver Nanoparticles Obtained from Moringa oleifera Seed Cake

João P. Jacinto, Rodrigo Silva, Jéssica C. Soares, Alice S. Pereira, Pedro Tavares

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In the present work, we report a simple, cost-efficient, and eco-friendly green method to synthesize silver nanoparticles with antimicrobial activity. An ethanolic extract from Moringa oleifera seed residue was used as a reducing and stabilizing agent in an aqueous solution of silver nitrate. The synthesized silver nanoparticles’ hydrodynamic radius, polydispersity index, and zeta-potential were evaluated by Dynamic Light Scattering. Scanning Electron Microscopy was employed to confirm the size and morphology of the nanoparticles. Synthesis of spherical particles with 127 ± 24 nm was confirmed. After sintering, the product of the synthesis was analyzed by X-ray diffraction. The X-ray diffraction pattern attributed to reflections of the (111), (200), (220), and (311) planes, which are characteristic of silver nanoparticles, confirms the successful synthesis of crystalline face-centered cubic nanoparticles. The antimicrobial activity of the bionanoparticles was tested against Escherichia coli BL21(DE3) cells and compared with the effect of a Moringa oleifera seed cake extract. Herein, we show that the growth of Escherichia coli is significantly affected by the addition of the synthesized bionanoparticles. Addition of the bionanoparticles inhibited the growth and lengthened the lag phase of the bacterial culture.
Original languageEnglish
Article number1439
Number of pages14
Issue number8
Publication statusPublished - 16 Aug 2023


  • antimicrobial activity
  • by-products valorization
  • green synthesis
  • Moringa oleifera seed cake
  • silver bionanoparticles


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