Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Tuberculosis, still one of the leading human infectious diseases, reported 8.7 million new cases in 2011 alone. Also, the increasing rate of multidrug-resistant tuberculosis (MDRTB) and its treatment difficulties pose a serious public health threat especially in developing countries. Resistance to isoniazid and rifampicin, first line antibiotics, is commonly associated with point mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis complex (MTBC). Therefore, the development of cheap, fast and simple molecular methods to assess susceptibility profiles would have a huge impact in the capacity of early diagnosis and treatment of MDRTB. Gold nanoparticles functionalized with thiol-modified oligonucleotides (Au-nanoprobes) have shown the potential to provide a rapid and sensitive detection method for MTBC and single base mutations associated with antibiotic resistance, namely the characterization of the three most relevant codons in rpoB gene associated to rifampicin resistance. Here we extend the Au-nanoprobe approach towards discriminating specific mutations within inhA and rpoB genes in PCR amplified DNA from isolates. Using a multiplex PCR reaction for these two genes, it is possible to assess both loci in parallel, and extend the potential of the Au-nanoprobe method to MDRTB molecular characterization with special application in the most frequent Portuguese genotypes. (C) 2014 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)332-337
Number of pages6
JournalTuberculosis
Volume94
Issue number3
DOIs
Publication statusPublished - May 2014

Fingerprint

Multidrug-Resistant Tuberculosis
Gold
Rifampin
Mycobacterium tuberculosis
Genes
Mutation
Multiplex Polymerase Chain Reaction
Isoniazid
Microbial Drug Resistance
Point Mutation
Sulfhydryl Compounds
Codon
Oligonucleotides
Nanoparticles
Developing Countries
Communicable Diseases
Early Diagnosis
Tuberculosis
Public Health
Genotype

Keywords

  • MDRTB
  • Nanodiagnostics
  • Gold nanoparticles
  • Tuberculosis
  • inhA
  • rpoB
  • MYCOBACTERIUM-TUBERCULOSIS
  • RAPID IDENTIFICATION
  • ASSAY
  • PLATFORM
  • COMPLEX
  • MUTATIONS
  • MECHANISM
  • GENE
  • DNA

Cite this

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title = "Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis",
abstract = "Tuberculosis, still one of the leading human infectious diseases, reported 8.7 million new cases in 2011 alone. Also, the increasing rate of multidrug-resistant tuberculosis (MDRTB) and its treatment difficulties pose a serious public health threat especially in developing countries. Resistance to isoniazid and rifampicin, first line antibiotics, is commonly associated with point mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis complex (MTBC). Therefore, the development of cheap, fast and simple molecular methods to assess susceptibility profiles would have a huge impact in the capacity of early diagnosis and treatment of MDRTB. Gold nanoparticles functionalized with thiol-modified oligonucleotides (Au-nanoprobes) have shown the potential to provide a rapid and sensitive detection method for MTBC and single base mutations associated with antibiotic resistance, namely the characterization of the three most relevant codons in rpoB gene associated to rifampicin resistance. Here we extend the Au-nanoprobe approach towards discriminating specific mutations within inhA and rpoB genes in PCR amplified DNA from isolates. Using a multiplex PCR reaction for these two genes, it is possible to assess both loci in parallel, and extend the potential of the Au-nanoprobe method to MDRTB molecular characterization with special application in the most frequent Portuguese genotypes. (C) 2014 Elsevier Ltd. All rights reserved.",
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Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis. / Pedrosa, Pedro; Veigas, Bruno; Machado, Diana; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro.

In: Tuberculosis, Vol. 94, No. 3, 05.2014, p. 332-337.

Research output: Contribution to journalArticle

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T1 - Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis

AU - Pedrosa, Pedro

AU - Veigas, Bruno

AU - Machado, Diana

AU - Couto, Isabel

AU - Viveiros, Miguel

AU - Baptista, Pedro

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