Abstract

The impact of advances in nanotechnology is particularly relevant in biodiagnostics, where nanoparticle-based assays have been developed for specific detection of bioanalytes of clinical interest. Gold nanoparticles show easily tuned physical properties, including unique optical properties, robustness, and high surface areas, making them ideal candidates for developing biomarker platforms. Modulation of these physicochemical properties can be easily achieved by adequate synthetic strategies and give gold nanoparticles advantages over conventional detection methods currently used in clinical diagnostics. The surface of gold nanoparticles can be tailored by ligand functionalization to selectively bind biomarkers. Thiol-linking of DNA and chemical functionalization of gold nanoparticles for specific protein/antibody binding are the most common approaches. Simple and inexpensive methods based on these bio-nanoprobes were initially applied for detection of specific DNA sequences and are presently being expanded to clinical diagnosis.
Original languageUnknown
Pages (from-to)943-950
JournalAnalytical and Bioanalytical Chemistry
Volume391
Issue number3
DOIs
Publication statusPublished - 1 Jan 2008

Cite this

@article{66dd366881d24e79ab4c23293ea01481,
title = "Gold nanoparticles for the development of clinical diagnosis methods",
abstract = "The impact of advances in nanotechnology is particularly relevant in biodiagnostics, where nanoparticle-based assays have been developed for specific detection of bioanalytes of clinical interest. Gold nanoparticles show easily tuned physical properties, including unique optical properties, robustness, and high surface areas, making them ideal candidates for developing biomarker platforms. Modulation of these physicochemical properties can be easily achieved by adequate synthetic strategies and give gold nanoparticles advantages over conventional detection methods currently used in clinical diagnostics. The surface of gold nanoparticles can be tailored by ligand functionalization to selectively bind biomarkers. Thiol-linking of DNA and chemical functionalization of gold nanoparticles for specific protein/antibody binding are the most common approaches. Simple and inexpensive methods based on these bio-nanoprobes were initially applied for detection of specific DNA sequences and are presently being expanded to clinical diagnosis.",
keywords = "Nanoparticles, gold nanoparticles, nanotechnology, nucleic acids (DNA/RNA), biological samples, bioanalytical methods",
author = "Baptista, {Pedro Miguel Ribeiro Viana} and Ricardo Franco",
year = "2008",
month = "1",
day = "1",
doi = "10.1007/s00216-007-1768-z",
language = "Unknown",
volume = "391",
pages = "943--950",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "SPRINGER HEIDELBERG",
number = "3",

}

TY - JOUR

T1 - Gold nanoparticles for the development of clinical diagnosis methods

AU - Baptista, Pedro Miguel Ribeiro Viana

AU - Franco, Ricardo

PY - 2008/1/1

Y1 - 2008/1/1

N2 - The impact of advances in nanotechnology is particularly relevant in biodiagnostics, where nanoparticle-based assays have been developed for specific detection of bioanalytes of clinical interest. Gold nanoparticles show easily tuned physical properties, including unique optical properties, robustness, and high surface areas, making them ideal candidates for developing biomarker platforms. Modulation of these physicochemical properties can be easily achieved by adequate synthetic strategies and give gold nanoparticles advantages over conventional detection methods currently used in clinical diagnostics. The surface of gold nanoparticles can be tailored by ligand functionalization to selectively bind biomarkers. Thiol-linking of DNA and chemical functionalization of gold nanoparticles for specific protein/antibody binding are the most common approaches. Simple and inexpensive methods based on these bio-nanoprobes were initially applied for detection of specific DNA sequences and are presently being expanded to clinical diagnosis.

AB - The impact of advances in nanotechnology is particularly relevant in biodiagnostics, where nanoparticle-based assays have been developed for specific detection of bioanalytes of clinical interest. Gold nanoparticles show easily tuned physical properties, including unique optical properties, robustness, and high surface areas, making them ideal candidates for developing biomarker platforms. Modulation of these physicochemical properties can be easily achieved by adequate synthetic strategies and give gold nanoparticles advantages over conventional detection methods currently used in clinical diagnostics. The surface of gold nanoparticles can be tailored by ligand functionalization to selectively bind biomarkers. Thiol-linking of DNA and chemical functionalization of gold nanoparticles for specific protein/antibody binding are the most common approaches. Simple and inexpensive methods based on these bio-nanoprobes were initially applied for detection of specific DNA sequences and are presently being expanded to clinical diagnosis.

KW - Nanoparticles

KW - gold nanoparticles

KW - nanotechnology

KW - nucleic acids (DNA/RNA)

KW - biological samples

KW - bioanalytical methods

U2 - 10.1007/s00216-007-1768-z

DO - 10.1007/s00216-007-1768-z

M3 - Article

VL - 391

SP - 943

EP - 950

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 3

ER -