Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

Hannah Winifred Child, Yulan Hernandez, Joao Conde, Margaret Mullin, Pedro Baptista, Jesus Maria de la Fuente, Catherine Cecilia Berry

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9 Citations (Scopus)

Abstract

Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

Original languageEnglish
Pages (from-to)2513-2525
Number of pages13
JournalNanomedicine
Volume10
Issue number16
DOIs
Publication statusPublished - 2015

Keywords

  • apoptosis
  • cell cycle
  • cell delivery
  • cell proliferation
  • c-myc
  • gene knockdown
  • gold nanoparticles
  • knockdown
  • nanoparticles
  • off-target effects
  • proliferation
  • RNAi
  • siRNA
  • C-MYC AMPLIFICATION
  • INTRACELLULAR DELIVERY
  • BREAST-CANCER
  • HUMAN-CELLS
  • IN-VITRO
  • INTERFERENCE
  • TARGET
  • GLUTATHIONE
  • DNA
  • THERAPY

Cite this

Child, H. W., Hernandez, Y., Conde, J., Mullin, M., Baptista, P., Maria de la Fuente, J., & Berry, C. C. (2015). Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects. Nanomedicine, 10(16), 2513-2525. https://doi.org/10.2217/NNM.15.95