Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

Hannah Winifred Child, Yulan Hernandez, Joao Conde, Margaret Mullin, Pedro Baptista, Jesus Maria de la Fuente, Catherine Cecilia Berry

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

Original languageEnglish
Pages (from-to)2513-2525
Number of pages13
JournalNanomedicine
Volume10
Issue number16
DOIs
Publication statusPublished - 2015

Keywords

  • apoptosis
  • cell cycle
  • cell delivery
  • cell proliferation
  • c-myc
  • gene knockdown
  • gold nanoparticles
  • knockdown
  • nanoparticles
  • off-target effects
  • proliferation
  • RNAi
  • siRNA
  • C-MYC AMPLIFICATION
  • INTRACELLULAR DELIVERY
  • BREAST-CANCER
  • HUMAN-CELLS
  • IN-VITRO
  • INTERFERENCE
  • TARGET
  • GLUTATHIONE
  • DNA
  • THERAPY

Cite this

Child, H. W., Hernandez, Y., Conde, J., Mullin, M., Baptista, P., Maria de la Fuente, J., & Berry, C. C. (2015). Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects. Nanomedicine, 10(16), 2513-2525. https://doi.org/10.2217/NNM.15.95
Child, Hannah Winifred ; Hernandez, Yulan ; Conde, Joao ; Mullin, Margaret ; Baptista, Pedro ; Maria de la Fuente, Jesus ; Berry, Catherine Cecilia. / Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects. In: Nanomedicine. 2015 ; Vol. 10, No. 16. pp. 2513-2525.
@article{ee4950e58a5d44be8740486be13a5294,
title = "Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects",
abstract = "Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.",
keywords = "apoptosis, cell cycle, cell delivery, cell proliferation, c-myc, gene knockdown, gold nanoparticles, knockdown, nanoparticles, off-target effects, proliferation, RNAi, siRNA, C-MYC AMPLIFICATION, INTRACELLULAR DELIVERY, BREAST-CANCER, HUMAN-CELLS, IN-VITRO, INTERFERENCE, TARGET, GLUTATHIONE, DNA, THERAPY",
author = "Child, {Hannah Winifred} and Yulan Hernandez and Joao Conde and Margaret Mullin and Pedro Baptista and {Maria de la Fuente}, Jesus and Berry, {Catherine Cecilia}",
year = "2015",
doi = "10.2217/NNM.15.95",
language = "English",
volume = "10",
pages = "2513--2525",
journal = "Nanomedicine",
issn = "1743-5889",
publisher = "FUTURE MEDICINE LTD",
number = "16",

}

Child, HW, Hernandez, Y, Conde, J, Mullin, M, Baptista, P, Maria de la Fuente, J & Berry, CC 2015, 'Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects' Nanomedicine, vol. 10, no. 16, pp. 2513-2525. https://doi.org/10.2217/NNM.15.95

Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects. / Child, Hannah Winifred; Hernandez, Yulan; Conde, Joao; Mullin, Margaret; Baptista, Pedro; Maria de la Fuente, Jesus; Berry, Catherine Cecilia.

In: Nanomedicine, Vol. 10, No. 16, 2015, p. 2513-2525.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

AU - Child, Hannah Winifred

AU - Hernandez, Yulan

AU - Conde, Joao

AU - Mullin, Margaret

AU - Baptista, Pedro

AU - Maria de la Fuente, Jesus

AU - Berry, Catherine Cecilia

PY - 2015

Y1 - 2015

N2 - Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

AB - Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

KW - apoptosis

KW - cell cycle

KW - cell delivery

KW - cell proliferation

KW - c-myc

KW - gene knockdown

KW - gold nanoparticles

KW - knockdown

KW - nanoparticles

KW - off-target effects

KW - proliferation

KW - RNAi

KW - siRNA

KW - C-MYC AMPLIFICATION

KW - INTRACELLULAR DELIVERY

KW - BREAST-CANCER

KW - HUMAN-CELLS

KW - IN-VITRO

KW - INTERFERENCE

KW - TARGET

KW - GLUTATHIONE

KW - DNA

KW - THERAPY

U2 - 10.2217/NNM.15.95

DO - 10.2217/NNM.15.95

M3 - Article

VL - 10

SP - 2513

EP - 2525

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 16

ER -