Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

Hannah Winifred Child; Yulan Hernandez; Joao Conde; Margaret Mullin; Pedro Baptista; Jesus Maria de la Fuente; Catherine Cecilia Berry

doi:10.2217/NNM.15.95

Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

Hannah Winifred Child, Yulan Hernandez, Joao Conde, Margaret Mullin, Pedro Baptista, Jesus Maria de la Fuente, Catherine Cecilia Berry

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

Original language	English
Pages (from-to)	2513-2525
Number of pages	13
Journal	Nanomedicine
Volume	10
Issue number	16
DOIs	https://doi.org/10.2217/NNM.15.95
Publication status	Published - 2015

Keywords

apoptosis
cell cycle
cell delivery
cell proliferation
c-myc
gene knockdown
gold nanoparticles
knockdown
nanoparticles
off-target effects
proliferation
RNAi
siRNA
C-MYC AMPLIFICATION
INTRACELLULAR DELIVERY
BREAST-CANCER
HUMAN-CELLS
IN-VITRO
INTERFERENCE
TARGET
GLUTATHIONE
DNA
THERAPY

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/NNM.15.95

Cite this

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title = "Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects",

abstract = "Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.",

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author = "Child, {Hannah Winifred} and Yulan Hernandez and Joao Conde and Margaret Mullin and Pedro Baptista and {Maria de la Fuente}, Jesus and Berry, {Catherine Cecilia}",

note = "Engineering & Physical Sciences Research Council (EPSRC) (EP/H006885/1)",

year = "2015",

doi = "10.2217/NNM.15.95",

language = "English",

volume = "10",

pages = "2513--2525",

journal = "Nanomedicine",

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TY - JOUR

T1 - Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

AU - Child, Hannah Winifred

AU - Hernandez, Yulan

AU - Conde, Joao

AU - Mullin, Margaret

AU - Baptista, Pedro

AU - Maria de la Fuente, Jesus

AU - Berry, Catherine Cecilia

N1 - Engineering & Physical Sciences Research Council (EPSRC) (EP/H006885/1)

PY - 2015

Y1 - 2015

N2 - Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

AB - Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.

KW - apoptosis

KW - cell cycle

KW - cell delivery

KW - cell proliferation

KW - c-myc

KW - gene knockdown

KW - gold nanoparticles

KW - knockdown

KW - nanoparticles

KW - off-target effects

KW - proliferation

KW - RNAi

KW - siRNA

KW - C-MYC AMPLIFICATION

KW - INTRACELLULAR DELIVERY

KW - BREAST-CANCER

KW - HUMAN-CELLS

KW - IN-VITRO

KW - INTERFERENCE

KW - TARGET

KW - GLUTATHIONE

KW - DNA

KW - THERAPY

U2 - 10.2217/NNM.15.95

DO - 10.2217/NNM.15.95

M3 - Article

C2 - 26302331

SN - 1743-5889

VL - 10

SP - 2513

EP - 2525

JO - Nanomedicine

JF - Nanomedicine

IS - 16

ER -

Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

Abstract

Keywords

UN SDGs

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