Gold-Nanobeacons as a theranostic system for the detection and inhibition of specific genes

Joao Conde, João Rosa, Pedro Baptista

Research output: Contribution to journalArticle

Abstract

This protocol describes the synthesis and detailed calibration of a gold nanoparticle-based nanobeacon (Au-nanobeacon) as an innovative theranostic approach for detection and inhibition of sequence-specific DNA and RNA for in vitro and ex vivo applications. Under hairpin configuration, proximity to gold nanoparticles leads to fluorescence quenching; hybridization to a complementary target restores fluorescence emission due to the gold nanobeacons’ conformational reorganization that causes the fluorophore and the AuNP to part from each other. This concept can easily be extended and adapted to assist the in vitro evaluation of silencing potential of a given sequence to be later used for ex vivo gene silencing and RNAi approaches, with the ability to monitor real-time gene delivery action. The time range for the entire protocol is ~8 days, including synthesis, functionalization and calibration of Au-nanobeacons, RNAi and gene silencing assays.
Original languageEnglish
JournalProtocol Exchange
DOIs
Publication statusPublished - 27 Nov 2013

Keywords

  • siRNA
  • gold nanoparticles
  • gold nanobeacons
  • gene silecning
  • miRNas
  • cell tracking

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